CRISPR-U™ Gene Knockout Cell Line
Strategy
HSPA8 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human HSPA8 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | HSPA8 |
| Gene id | 3312 |
| Organism | Homo sapiens |
| Official full symbol | heat shock protein family A (Hsp70) member 8 |
| Gene type | protein-coding |
| Also known as | HEL-33, HEL-S-72p, HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP-1, LAP1, NIP71 |
| Summary | This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 11 |
Strategy Summary
This gene has 17 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| HSPA8-218 | ENST00000532636.5 | 2306 | 646aa | Protein coding | CCDS8440 | P11142-1 | - | TSL:5, GENCODE basic, APPRIS P1, |
| HSPA8-224 | ENST00000534624.6 | 2264 | 646aa | Protein coding | CCDS8440 | P11142-1 | NM_006597.6 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| HSPA8-201 | ENST00000227378.7 | 2186 | 646aa | Protein coding | CCDS8440 | P11142-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| HSPA8-202 | ENST00000453788.6 | 2004 | 493aa | Protein coding | CCDS44754 | P11142-2 | - | TSL:1, GENCODE basic, |
| HSPA8-207 | ENST00000526110.5 | 1924 | 627aa | Protein coding | - | E9PKE3 | - | TSL:5, GENCODE basic, |
| HSPA8-222 | ENST00000534319.5 | 1884 | 410aa | Protein coding | - | A8K7Q2 | - | TSL:2, GENCODE basic, |
| HSPA8-221 | ENST00000533540.5 | 1826 | 500aa | Protein coding | - | E9PNE6 | - | TSL:5, GENCODE basic, |
| HSPA8-212 | ENST00000528292.5 | 1112 | 312aa | Protein coding | - | E9PN89 | - | CDS 3' incomplete, TSL:2, |
| HSPA8-206 | ENST00000525624.5 | 1048 | 187aa | Protein coding | - | E9PLF4 | - | CDS 3' incomplete, TSL:5, |
| HSPA8-203 | ENST00000524552.5 | 963 | 223aa | Protein coding | - | E9PS65 | - | CDS 3' incomplete, TSL:1, |
| HSPA8-208 | ENST00000526686.1 | 740 | 150aa | Protein coding | - | E9PM13 | - | CDS 3' incomplete, TSL:1, |
| HSPA8-217 | ENST00000532182.5 | 674 | 171aa | Protein coding | - | E9PQQ4 | - | CDS 3' incomplete, TSL:4, |
| HSPA8-213 | ENST00000530391.1 | 648 | 132aa | Protein coding | - | E9PN25 | - | CDS 3' incomplete, TSL:3, |
| HSPA8-223 | ENST00000534567.5 | 588 | 183aa | Protein coding | - | E9PK54 | - | CDS 3' incomplete, TSL:4, |
| HSPA8-205 | ENST00000525463.5 | 583 | 168aa | Protein coding | - | E9PI65 | - | CDS 3' incomplete, TSL:5, |
| HSPA8-210 | ENST00000527387.5 | 579 | 178aa | Protein coding | - | E9PQK7 | - | CDS 3' incomplete, TSL:4, |
| HSPA8-204 | ENST00000524590.5 | 536 | 137aa | Protein coding | - | E9PPY6 | - | CDS 3' incomplete, TSL:5, |
| HSPA8-209 | ENST00000526862.1 | 504 | No protein | Processed transcript | - | - | - | TSL:3, |
| HSPA8-220 | ENST00000533238.5 | 465 | No protein | Processed transcript | - | - | - | TSL:3, |
| HSPA8-215 | ENST00000532091.1 | 2301 | No protein | Retained intron | - | - | - | TSL:5, |
| HSPA8-211 | ENST00000527983.5 | 1509 | No protein | Retained intron | - | - | - | TSL:1, |
| HSPA8-219 | ENST00000532780.5 | 1194 | No protein | Retained intron | - | - | - | TSL:2, |
| HSPA8-214 | ENST00000531063.1 | 578 | No protein | Retained intron | - | - | - | TSL:2, |
| HSPA8-216 | ENST00000532167.5 | 570 | No protein | Retained intron | - | - | - | TSL:4, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | HSPA8 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene HSPA8 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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