CRISPR-U™ Gene Knockout Cell Line Strategy

HSPA8 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human HSPA8 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol HSPA8
Gene id 3312
Organism Homo sapiens
Official full symbol heat shock protein family A (Hsp70) member 8
Gene type protein-coding
Also known as HEL-33, HEL-S-72p, HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP-1, LAP1, NIP71
Summary This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 11
Strategy Summary
This gene has 17 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
HSPA8-218 ENST00000532636.5 2306 646aa Protein coding CCDS8440 P11142-1 - TSL:5, GENCODE basic, APPRIS P1,
HSPA8-224 ENST00000534624.6 2264 646aa Protein coding CCDS8440 P11142-1 NM_006597.6 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
HSPA8-201 ENST00000227378.7 2186 646aa Protein coding CCDS8440 P11142-1 - TSL:1, GENCODE basic, APPRIS P1,
HSPA8-202 ENST00000453788.6 2004 493aa Protein coding CCDS44754 P11142-2 - TSL:1, GENCODE basic,
HSPA8-207 ENST00000526110.5 1924 627aa Protein coding - E9PKE3 - TSL:5, GENCODE basic,
HSPA8-222 ENST00000534319.5 1884 410aa Protein coding - A8K7Q2 - TSL:2, GENCODE basic,
HSPA8-221 ENST00000533540.5 1826 500aa Protein coding - E9PNE6 - TSL:5, GENCODE basic,
HSPA8-212 ENST00000528292.5 1112 312aa Protein coding - E9PN89 - CDS 3' incomplete, TSL:2,
HSPA8-206 ENST00000525624.5 1048 187aa Protein coding - E9PLF4 - CDS 3' incomplete, TSL:5,
HSPA8-203 ENST00000524552.5 963 223aa Protein coding - E9PS65 - CDS 3' incomplete, TSL:1,
HSPA8-208 ENST00000526686.1 740 150aa Protein coding - E9PM13 - CDS 3' incomplete, TSL:1,
HSPA8-217 ENST00000532182.5 674 171aa Protein coding - E9PQQ4 - CDS 3' incomplete, TSL:4,
HSPA8-213 ENST00000530391.1 648 132aa Protein coding - E9PN25 - CDS 3' incomplete, TSL:3,
HSPA8-223 ENST00000534567.5 588 183aa Protein coding - E9PK54 - CDS 3' incomplete, TSL:4,
HSPA8-205 ENST00000525463.5 583 168aa Protein coding - E9PI65 - CDS 3' incomplete, TSL:5,
HSPA8-210 ENST00000527387.5 579 178aa Protein coding - E9PQK7 - CDS 3' incomplete, TSL:4,
HSPA8-204 ENST00000524590.5 536 137aa Protein coding - E9PPY6 - CDS 3' incomplete, TSL:5,
HSPA8-209 ENST00000526862.1 504 No protein Processed transcript - - - TSL:3,
HSPA8-220 ENST00000533238.5 465 No protein Processed transcript - - - TSL:3,
HSPA8-215 ENST00000532091.1 2301 No protein Retained intron - - - TSL:5,
HSPA8-211 ENST00000527983.5 1509 No protein Retained intron - - - TSL:1,
HSPA8-219 ENST00000532780.5 1194 No protein Retained intron - - - TSL:2,
HSPA8-214 ENST00000531063.1 578 No protein Retained intron - - - TSL:2,
HSPA8-216 ENST00000532167.5 570 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene HSPA8
This KO Strategy loading
Red Cotton™ Notes Gene HSPA8 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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