CRISPR-U™ Gene Knockout Cell Line Strategy

DNAJA1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human DNAJA1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol DNAJA1
Gene id 3301
Organism Homo sapiens
Official full symbol DnaJ heat shock protein family (Hsp40) member A1
Gene type protein-coding
Also known as DJ-2, DjA1, HDJ2, HSDJ, HSJ-2, HSJ2, HSPF4, NEDD7, hDJ-2
Summary This gene encodes a member of the DnaJ family of proteins, which act as heat shock protein 70 cochaperones. Heat shock proteins facilitate protein folding, trafficking, prevention of aggregation, and proteolytic degradation. Members of this family are characterized by a highly conserved N-terminal J domain, a glycine/phenylalanine-rich region, four CxxCxGxG zinc finger repeats, and a C-terminal substrate-binding domain. The J domain mediates the interaction with heat shock protein 70 to recruit substrates and regulate ATP hydrolysis activity. In humans, this gene has been implicated in positive regulation of virus replication through co-option by the influenza A virus. Several pseudogenes of this gene are found on other chromosomes.
Genomic regions Chromosome 9
Strategy Summary
This gene has 1 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
DNAJA1-201 ENST00000330899.5 2319 397aa Protein coding CCDS6533 P31689-1 NM_001539.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
DNAJA1-203 ENST00000495015.5 604 No protein Processed transcript - - - TSL:3,
DNAJA1-202 ENST00000465677.1 505 No protein Processed transcript - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene DNAJA1
This KO Strategy loading
Red Cotton™ Notes Gene DNAJA1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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