CRISPR-U™ Gene Knockout Cell Line
Strategy
DNAJA1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human DNAJA1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | DNAJA1 |
| Gene id | 3301 |
| Organism | Homo sapiens |
| Official full symbol | DnaJ heat shock protein family (Hsp40) member A1 |
| Gene type | protein-coding |
| Also known as | DJ-2, DjA1, HDJ2, HSDJ, HSJ-2, HSJ2, HSPF4, NEDD7, hDJ-2 |
| Summary | This gene encodes a member of the DnaJ family of proteins, which act as heat shock protein 70 cochaperones. Heat shock proteins facilitate protein folding, trafficking, prevention of aggregation, and proteolytic degradation. Members of this family are characterized by a highly conserved N-terminal J domain, a glycine/phenylalanine-rich region, four CxxCxGxG zinc finger repeats, and a C-terminal substrate-binding domain. The J domain mediates the interaction with heat shock protein 70 to recruit substrates and regulate ATP hydrolysis activity. In humans, this gene has been implicated in positive regulation of virus replication through co-option by the influenza A virus. Several pseudogenes of this gene are found on other chromosomes. |
| Genomic regions | Chromosome 9 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| DNAJA1-201 | ENST00000330899.5 | 2319 | 397aa | Protein coding | CCDS6533 | P31689-1 | NM_001539.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| DNAJA1-203 | ENST00000495015.5 | 604 | No protein | Processed transcript | - | - | - | TSL:3, |
| DNAJA1-202 | ENST00000465677.1 | 505 | No protein | Processed transcript | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | DNAJA1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene DNAJA1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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