CRISPR-U™ Gene Knockout Cell Line
Strategy
HNRNPAB Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human HNRNPAB Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | HNRNPAB |
| Gene id | 3182 |
| Organism | Homo sapiens |
| Official full symbol | heterogeneous nuclear ribonucleoprotein A/B |
| Gene type | protein-coding |
| Also known as | ABBP1, HNRPAB |
| Summary | This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are produced by RNA polymerase II and are components of the heterogeneous nuclear RNA (hnRNA) complexes. They are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene, which binds to one of the components of the multiprotein editosome complex, has two repeats of quasi-RRM (RNA recognition motif) domains that bind to RNAs. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. |
| Genomic regions | Chromosome 5 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| HNRNPAB-204 | ENST00000504898.5 | 1785 | 332aa | Protein coding | CCDS34309 | Q99729-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| HNRNPAB-202 | ENST00000358344.8 | 1770 | 332aa | Protein coding | CCDS34309 | Q99729-2 | NM_031266.3 | TSL:1, GENCODE basic, APPRIS ALT2, MANE Select v0.92, |
| HNRNPAB-205 | ENST00000506259.5 | 1674 | 285aa | Protein coding | CCDS34310 | Q99729-3 | - | TSL:1, GENCODE basic, APPRIS P3, |
| HNRNPAB-201 | ENST00000355836.9 | 1602 | 285aa | Protein coding | CCDS34310 | Q99729-3 | - | TSL:1, GENCODE basic, APPRIS P3, |
| HNRNPAB-206 | ENST00000506339.5 | 1747 | 327aa | Protein coding | - | D6RBZ0 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| HNRNPAB-208 | ENST00000515193.5 | 1595 | 280aa | Protein coding | - | D6R9P3 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| HNRNPAB-207 | ENST00000514633.5 | 1571 | 283aa | Protein coding | - | D6RD18 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| HNRNPAB-203 | ENST00000504796.1 | 578 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | HNRNPAB |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene HNRNPAB had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: