CRISPR-U™ Gene Knockout Cell Line
Strategy
HNRNPA2B1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human HNRNPA2B1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | HNRNPA2B1 |
| Gene id | 3181 |
| Organism | Homo sapiens |
| Official full symbol | heterogeneous nuclear ribonucleoprotein A2/B1 |
| Gene type | protein-coding |
| Also known as | HNRNPA2, HNRNPB1, HNRPA2, HNRPA2B1, HNRPB1, IBMPFD2, RNPA2, SNRPB1 |
| Summary | This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. This gene has been described to generate two alternatively spliced transcript variants which encode different isoforms. |
| Genomic regions | Chromosome 7 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| HNRNPA2B1-226 | ENST00000677839.1 | 4333 | 341aa | Protein coding | CCDS5397 | - | - | GENCODE basic, APPRIS ALT1, |
| HNRNPA2B1-202 | ENST00000356674.8 | 4106 | 353aa | Protein coding | CCDS43557 | P22626-2 | - | TSL:1, GENCODE basic, APPRIS ALT1, |
| HNRNPA2B1-201 | ENST00000354667.8 | 3664 | 353aa | Protein coding | CCDS43557 | P22626-1 | - | TSL:1, GENCODE basic, APPRIS P3, |
| HNRNPA2B1-209 | ENST00000618183.5 | 3628 | 341aa | Protein coding | CCDS5397 | A0A087WUI2 | NM_002137.4 | TSL:5, GENCODE basic, APPRIS ALT1, MANE Select v0.92, |
| HNRNPA2B1-208 | ENST00000608362.2 | 3497 | 341aa | Protein coding | CCDS5397 | P22626-2 | - | TSL:NA, GENCODE basic, APPRIS ALT1, |
| HNRNPA2B1-213 | ENST00000676749.1 | 2978 | 341aa | Protein coding | CCDS5397 | - | - | GENCODE basic, APPRIS ALT1, |
| HNRNPA2B1-222 | ENST00000677574.1 | 4092 | 341aa | Nonsense mediated decay | CCDS5397 | - | - | - |
| HNRNPA2B1-227 | ENST00000677906.1 | 4068 | 341aa | Nonsense mediated decay | CCDS5397 | - | - | - |
| HNRNPA2B1-246 | ENST00000679123.1 | 4044 | 353aa | Nonsense mediated decay | CCDS43557 | - | - | - |
| HNRNPA2B1-245 | ENST00000679021.1 | 3850 | 353aa | Nonsense mediated decay | CCDS43557 | - | - | - |
| HNRNPA2B1-233 | ENST00000678449.1 | 3754 | 341aa | Nonsense mediated decay | CCDS5397 | - | - | - |
| HNRNPA2B1-219 | ENST00000677339.1 | 3752 | 341aa | Nonsense mediated decay | CCDS5397 | - | - | - |
| HNRNPA2B1-241 | ENST00000678962.1 | 3730 | 341aa | Nonsense mediated decay | CCDS5397 | - | - | - |
| HNRNPA2B1-244 | ENST00000679001.1 | 3706 | 353aa | Nonsense mediated decay | CCDS43557 | - | - | - |
| HNRNPA2B1-234 | ENST00000678501.1 | 3677 | 341aa | Nonsense mediated decay | CCDS5397 | - | - | - |
| HNRNPA2B1-248 | ENST00000679243.1 | 3622 | 341aa | Nonsense mediated decay | CCDS5397 | - | - | - |
| HNRNPA2B1-232 | ENST00000678431.1 | 3522 | 341aa | Nonsense mediated decay | CCDS5397 | - | - | - |
| HNRNPA2B1-224 | ENST00000677656.1 | 3273 | 341aa | Nonsense mediated decay | CCDS5397 | - | - | - |
| HNRNPA2B1-214 | ENST00000676903.1 | 3258 | 341aa | Nonsense mediated decay | CCDS5397 | - | - | - |
| HNRNPA2B1-223 | ENST00000677631.1 | 3064 | 341aa | Nonsense mediated decay | CCDS5397 | - | - | - |
| HNRNPA2B1-211 | ENST00000676524.1 | 2949 | 341aa | Nonsense mediated decay | CCDS5397 | - | - | - |
| HNRNPA2B1-210 | ENST00000676497.1 | 2870 | 341aa | Nonsense mediated decay | CCDS5397 | - | - | - |
| HNRNPA2B1-236 | ENST00000678675.1 | 2731 | 353aa | Nonsense mediated decay | CCDS43557 | - | - | - |
| HNRNPA2B1-240 | ENST00000678935.1 | 2656 | 341aa | Nonsense mediated decay | CCDS5397 | - | - | - |
| HNRNPA2B1-243 | ENST00000678998.1 | 2612 | 341aa | Nonsense mediated decay | CCDS5397 | - | - | - |
| HNRNPA2B1-220 | ENST00000677396.1 | 2577 | 341aa | Nonsense mediated decay | CCDS5397 | - | - | - |
| HNRNPA2B1-237 | ENST00000678697.1 | 2463 | 341aa | Nonsense mediated decay | CCDS5397 | - | - | - |
| HNRNPA2B1-212 | ENST00000676746.1 | 2438 | 353aa | Nonsense mediated decay | CCDS43557 | - | - | - |
| HNRNPA2B1-238 | ENST00000678779.1 | 2348 | 341aa | Nonsense mediated decay | CCDS5397 | - | - | - |
| HNRNPA2B1-249 | ENST00000679318.1 | 2271 | 341aa | Nonsense mediated decay | CCDS5397 | - | - | - |
| HNRNPA2B1-239 | ENST00000678884.1 | 2224 | 341aa | Nonsense mediated decay | CCDS5397 | - | - | - |
| HNRNPA2B1-203 | ENST00000360787.8 | 1724 | 353aa | Nonsense mediated decay | CCDS43557 | P22626-1 | - | TSL:5, |
| HNRNPA2B1-229 | ENST00000678075.1 | 4328 | 301aa | Nonsense mediated decay | - | A0A024RA28 | - | - |
| HNRNPA2B1-228 | ENST00000678035.1 | 4172 | 301aa | Nonsense mediated decay | - | A0A024RA28 | - | - |
| HNRNPA2B1-231 | ENST00000678277.1 | 3907 | 158aa | Nonsense mediated decay | - | - | - | - |
| HNRNPA2B1-247 | ENST00000679124.1 | 3888 | 333aa | Nonsense mediated decay | - | - | - | - |
| HNRNPA2B1-221 | ENST00000677571.1 | 3829 | 325aa | Nonsense mediated decay | - | - | - | - |
| HNRNPA2B1-242 | ENST00000678973.1 | 3816 | 309aa | Nonsense mediated decay | - | - | - | - |
| HNRNPA2B1-218 | ENST00000677321.1 | 3792 | 301aa | Nonsense mediated decay | - | - | - | - |
| HNRNPA2B1-230 | ENST00000678183.1 | 3589 | 318aa | Nonsense mediated decay | - | - | - | - |
| HNRNPA2B1-235 | ENST00000678631.1 | 2416 | 260aa | Nonsense mediated decay | - | - | - | - |
| HNRNPA2B1-216 | ENST00000677037.1 | 2262 | 155aa | Nonsense mediated decay | - | - | - | - |
| HNRNPA2B1-206 | ENST00000490912.6 | 6718 | No protein | Retained intron | - | - | - | TSL:2, |
| HNRNPA2B1-204 | ENST00000463181.5 | 6284 | No protein | Retained intron | - | - | - | TSL:2, |
| HNRNPA2B1-205 | ENST00000476233.2 | 4942 | No protein | Retained intron | - | - | - | TSL:1, |
| HNRNPA2B1-217 | ENST00000677075.1 | 4469 | No protein | Retained intron | - | - | - | - |
| HNRNPA2B1-215 | ENST00000676932.1 | 4269 | No protein | Retained intron | - | - | - | - |
| HNRNPA2B1-225 | ENST00000677669.1 | 4060 | No protein | Retained intron | - | - | - | - |
| HNRNPA2B1-207 | ENST00000495810.2 | 1580 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | HNRNPA2B1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene HNRNPA2B1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
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Work flow
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