CRISPR-U™ Gene Knockout Cell Line Strategy

HNRNPA2B1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human HNRNPA2B1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol HNRNPA2B1
Gene id 3181
Organism Homo sapiens
Official full symbol heterogeneous nuclear ribonucleoprotein A2/B1
Gene type protein-coding
Also known as HNRNPA2, HNRNPB1, HNRPA2, HNRPA2B1, HNRPB1, IBMPFD2, RNPA2, SNRPB1
Summary This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. This gene has been described to generate two alternatively spliced transcript variants which encode different isoforms.
Genomic regions Chromosome 7
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
HNRNPA2B1-226 ENST00000677839.1 4333 341aa Protein coding CCDS5397 - - GENCODE basic, APPRIS ALT1,
HNRNPA2B1-202 ENST00000356674.8 4106 353aa Protein coding CCDS43557 P22626-2 - TSL:1, GENCODE basic, APPRIS ALT1,
HNRNPA2B1-201 ENST00000354667.8 3664 353aa Protein coding CCDS43557 P22626-1 - TSL:1, GENCODE basic, APPRIS P3,
HNRNPA2B1-209 ENST00000618183.5 3628 341aa Protein coding CCDS5397 A0A087WUI2 NM_002137.4 TSL:5, GENCODE basic, APPRIS ALT1, MANE Select v0.92,
HNRNPA2B1-208 ENST00000608362.2 3497 341aa Protein coding CCDS5397 P22626-2 - TSL:NA, GENCODE basic, APPRIS ALT1,
HNRNPA2B1-213 ENST00000676749.1 2978 341aa Protein coding CCDS5397 - - GENCODE basic, APPRIS ALT1,
HNRNPA2B1-222 ENST00000677574.1 4092 341aa Nonsense mediated decay CCDS5397 - - -
HNRNPA2B1-227 ENST00000677906.1 4068 341aa Nonsense mediated decay CCDS5397 - - -
HNRNPA2B1-246 ENST00000679123.1 4044 353aa Nonsense mediated decay CCDS43557 - - -
HNRNPA2B1-245 ENST00000679021.1 3850 353aa Nonsense mediated decay CCDS43557 - - -
HNRNPA2B1-233 ENST00000678449.1 3754 341aa Nonsense mediated decay CCDS5397 - - -
HNRNPA2B1-219 ENST00000677339.1 3752 341aa Nonsense mediated decay CCDS5397 - - -
HNRNPA2B1-241 ENST00000678962.1 3730 341aa Nonsense mediated decay CCDS5397 - - -
HNRNPA2B1-244 ENST00000679001.1 3706 353aa Nonsense mediated decay CCDS43557 - - -
HNRNPA2B1-234 ENST00000678501.1 3677 341aa Nonsense mediated decay CCDS5397 - - -
HNRNPA2B1-248 ENST00000679243.1 3622 341aa Nonsense mediated decay CCDS5397 - - -
HNRNPA2B1-232 ENST00000678431.1 3522 341aa Nonsense mediated decay CCDS5397 - - -
HNRNPA2B1-224 ENST00000677656.1 3273 341aa Nonsense mediated decay CCDS5397 - - -
HNRNPA2B1-214 ENST00000676903.1 3258 341aa Nonsense mediated decay CCDS5397 - - -
HNRNPA2B1-223 ENST00000677631.1 3064 341aa Nonsense mediated decay CCDS5397 - - -
HNRNPA2B1-211 ENST00000676524.1 2949 341aa Nonsense mediated decay CCDS5397 - - -
HNRNPA2B1-210 ENST00000676497.1 2870 341aa Nonsense mediated decay CCDS5397 - - -
HNRNPA2B1-236 ENST00000678675.1 2731 353aa Nonsense mediated decay CCDS43557 - - -
HNRNPA2B1-240 ENST00000678935.1 2656 341aa Nonsense mediated decay CCDS5397 - - -
HNRNPA2B1-243 ENST00000678998.1 2612 341aa Nonsense mediated decay CCDS5397 - - -
HNRNPA2B1-220 ENST00000677396.1 2577 341aa Nonsense mediated decay CCDS5397 - - -
HNRNPA2B1-237 ENST00000678697.1 2463 341aa Nonsense mediated decay CCDS5397 - - -
HNRNPA2B1-212 ENST00000676746.1 2438 353aa Nonsense mediated decay CCDS43557 - - -
HNRNPA2B1-238 ENST00000678779.1 2348 341aa Nonsense mediated decay CCDS5397 - - -
HNRNPA2B1-249 ENST00000679318.1 2271 341aa Nonsense mediated decay CCDS5397 - - -
HNRNPA2B1-239 ENST00000678884.1 2224 341aa Nonsense mediated decay CCDS5397 - - -
HNRNPA2B1-203 ENST00000360787.8 1724 353aa Nonsense mediated decay CCDS43557 P22626-1 - TSL:5,
HNRNPA2B1-229 ENST00000678075.1 4328 301aa Nonsense mediated decay - A0A024RA28 - -
HNRNPA2B1-228 ENST00000678035.1 4172 301aa Nonsense mediated decay - A0A024RA28 - -
HNRNPA2B1-231 ENST00000678277.1 3907 158aa Nonsense mediated decay - - - -
HNRNPA2B1-247 ENST00000679124.1 3888 333aa Nonsense mediated decay - - - -
HNRNPA2B1-221 ENST00000677571.1 3829 325aa Nonsense mediated decay - - - -
HNRNPA2B1-242 ENST00000678973.1 3816 309aa Nonsense mediated decay - - - -
HNRNPA2B1-218 ENST00000677321.1 3792 301aa Nonsense mediated decay - - - -
HNRNPA2B1-230 ENST00000678183.1 3589 318aa Nonsense mediated decay - - - -
HNRNPA2B1-235 ENST00000678631.1 2416 260aa Nonsense mediated decay - - - -
HNRNPA2B1-216 ENST00000677037.1 2262 155aa Nonsense mediated decay - - - -
HNRNPA2B1-206 ENST00000490912.6 6718 No protein Retained intron - - - TSL:2,
HNRNPA2B1-204 ENST00000463181.5 6284 No protein Retained intron - - - TSL:2,
HNRNPA2B1-205 ENST00000476233.2 4942 No protein Retained intron - - - TSL:1,
HNRNPA2B1-217 ENST00000677075.1 4469 No protein Retained intron - - - -
HNRNPA2B1-215 ENST00000676932.1 4269 No protein Retained intron - - - -
HNRNPA2B1-225 ENST00000677669.1 4060 No protein Retained intron - - - -
HNRNPA2B1-207 ENST00000495810.2 1580 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene HNRNPA2B1
This KO Strategy loading
Red Cotton™ Notes Gene HNRNPA2B1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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