CRISPR-U™ Gene Knockout Cell Line
Strategy
NUDT2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NUDT2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NUDT2 |
| Gene id | 318 |
| Organism | Homo sapiens |
| Official full symbol | nudix hydrolase 2 |
| Gene type | protein-coding |
| Also known as | APAH1 |
| Summary | This gene encodes a member of the MutT family of nucleotide pyrophosphatases, a subset of the larger NUDIX hydrolase family. The gene product possesses a modification of the MutT sequence motif found in certain nucleotide pyrophosphatases. The enzyme asymmetrically hydrolyzes Ap4A to yield AMP and ATP and is responsible for maintaining the intracellular level of the dinucleotide Ap4A, the function of which has yet to be established. This gene may be a candidate tumor suppressor gene. Alternative splicing has been observed at this locus and four transcript variants, all encoding the same protein, have been identified. |
| Genomic regions | Chromosome 9 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NUDT2-202 | ENST00000379155.9 | 1002 | 147aa | Protein coding | CCDS6552 | P50583 | - | TSL:3, GENCODE basic, APPRIS P1, |
| NUDT2-203 | ENST00000379158.7 | 998 | 147aa | Protein coding | CCDS6552 | P50583 | NM_001161.5 | TSL:3, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| NUDT2-201 | ENST00000346365.8 | 960 | 147aa | Protein coding | CCDS6552 | P50583 | - | TSL:1, GENCODE basic, APPRIS P1, |
| NUDT2-204 | ENST00000618590.1 | 825 | 147aa | Protein coding | CCDS6552 | P50583 | - | TSL:3, GENCODE basic, APPRIS P1, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NUDT2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NUDT2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
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$599
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Work flow
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