CRISPR-U™ Gene Knockout Cell Line Strategy

NUDT2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human NUDT2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NUDT2
Gene id 318
Organism Homo sapiens
Official full symbol nudix hydrolase 2
Gene type protein-coding
Also known as APAH1
Summary This gene encodes a member of the MutT family of nucleotide pyrophosphatases, a subset of the larger NUDIX hydrolase family. The gene product possesses a modification of the MutT sequence motif found in certain nucleotide pyrophosphatases. The enzyme asymmetrically hydrolyzes Ap4A to yield AMP and ATP and is responsible for maintaining the intracellular level of the dinucleotide Ap4A, the function of which has yet to be established. This gene may be a candidate tumor suppressor gene. Alternative splicing has been observed at this locus and four transcript variants, all encoding the same protein, have been identified.
Genomic regions Chromosome 9
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NUDT2-202 ENST00000379155.9 1002 147aa Protein coding CCDS6552 P50583 - TSL:3, GENCODE basic, APPRIS P1,
NUDT2-203 ENST00000379158.7 998 147aa Protein coding CCDS6552 P50583 NM_001161.5 TSL:3, GENCODE basic, APPRIS P1, MANE Select v0.92,
NUDT2-201 ENST00000346365.8 960 147aa Protein coding CCDS6552 P50583 - TSL:1, GENCODE basic, APPRIS P1,
NUDT2-204 ENST00000618590.1 825 147aa Protein coding CCDS6552 P50583 - TSL:3, GENCODE basic, APPRIS P1,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NUDT2
This KO Strategy loading
Red Cotton™ Notes Gene NUDT2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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