CRISPR-U™ Gene Knockout Cell Line Strategy

FOXA2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human FOXA2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol FOXA2
Gene id 3170
Organism Homo sapiens
Official full symbol forkhead box A2
Gene type protein-coding
Also known as HNF-3-beta, HNF3B, TCF3B
Summary This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific genes such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young. Transcript variants encoding different isoforms have been identified for this gene.
Genomic regions Chromosome 20
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
FOXA2-202 ENST00000419308.7 2538 463aa Protein coding CCDS46585 Q9Y261-2 NM_021784.5 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
FOXA2-201 ENST00000377115.4 2401 457aa Protein coding CCDS13147 Q9Y261-1 - TSL:1, GENCODE basic, APPRIS ALT1,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene FOXA2
This KO Strategy loading
Red Cotton™ Notes Gene FOXA2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$39

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×

Comment: