CRISPR-U™ Gene Knockout Cell Line
Strategy
NR4A1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NR4A1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NR4A1 |
| Gene id | 3164 |
| Organism | Homo sapiens |
| Official full symbol | nuclear receptor subfamily 4 group A member 1 |
| Gene type | protein-coding |
| Also known as | GFRP1, HMR, N10, NAK-1, NGFIB, NP10, NUR77, TR3 |
| Summary | This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 12 |
Strategy Summary
This gene has 13 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NR4A1-206 | ENST00000545748.5 | 3511 | 652aa | Protein coding | CCDS73471 | F5GXF0 | - | TSL:2, GENCODE basic, APPRIS ALT2, |
| NR4A1-201 | ENST00000243050.5 | 2678 | 598aa | Protein coding | CCDS8818 | P22736-1 | - | TSL:1, GENCODE basic, APPRIS P2, |
| NR4A1-203 | ENST00000394824.2 | 2639 | 598aa | Protein coding | CCDS8818 | P22736-1 | - | TSL:5, GENCODE basic, APPRIS P2, |
| NR4A1-202 | ENST00000360284.7 | 2589 | 611aa | Protein coding | CCDS55828 | P22736-2 | - | TSL:2, GENCODE basic, APPRIS ALT2, |
| NR4A1-204 | ENST00000394825.6 | 2485 | 598aa | Protein coding | CCDS8818 | P22736-1 | NM_173157.3 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| NR4A1-213 | ENST00000550082.5 | 2029 | 611aa | Protein coding | CCDS55828 | P22736-2 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| NR4A1-207 | ENST00000546842.5 | 1258 | 302aa | Protein coding | - | H3BT85 | - | CDS 3' incomplete, TSL:2, |
| NR4A1-209 | ENST00000548232.1 | 1101 | 325aa | Protein coding | - | P22736-3 | - | TSL:1, GENCODE basic, |
| NR4A1-219 | ENST00000562373.1 | 838 | 150aa | Protein coding | - | H3BPN8 | - | CDS 3' incomplete, TSL:3, |
| NR4A1-216 | ENST00000550582.2 | 607 | 120aa | Protein coding | - | H3BQG5 | - | CDS 3' incomplete, TSL:5, |
| NR4A1-217 | ENST00000550763.1 | 574 | 148aa | Protein coding | - | F8VVX9 | - | CDS 3' incomplete, TSL:4, |
| NR4A1-211 | ENST00000548977.5 | 548 | 119aa | Protein coding | - | H3BSB9 | - | CDS 3' incomplete, TSL:2, |
| NR4A1-218 | ENST00000553200.1 | 521 | 78aa | Protein coding | - | H3BSM8 | - | CDS 3' incomplete, TSL:3, |
| NR4A1-208 | ENST00000547206.5 | 570 | No protein | Processed transcript | - | - | - | TSL:4, |
| NR4A1-210 | ENST00000548733.1 | 535 | No protein | Processed transcript | - | - | - | TSL:3, |
| NR4A1-215 | ENST00000550557.1 | 4837 | No protein | Retained intron | - | - | - | TSL:1, |
| NR4A1-205 | ENST00000478250.1 | 1820 | No protein | Retained intron | - | - | - | TSL:1, |
| NR4A1-220 | ENST00000564201.1 | 752 | No protein | Retained intron | - | - | - | TSL:5, |
| NR4A1-221 | ENST00000565848.1 | 691 | No protein | Retained intron | - | - | - | TSL:2, |
| NR4A1-222 | ENST00000567890.1 | 612 | No protein | Retained intron | - | - | - | TSL:5, |
| NR4A1-212 | ENST00000549102.1 | 600 | No protein | Retained intron | - | - | - | TSL:1, |
| NR4A1-214 | ENST00000550339.1 | 500 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NR4A1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NR4A1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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