CRISPR-U™ Gene Knockout Cell Line Strategy

HMGN2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human HMGN2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol HMGN2
Gene id 3151
Organism Homo sapiens
Official full symbol high mobility group nucleosomal binding domain 2
Gene type protein-coding
Also known as HMG17
Summary The protein encoded by this gene binds nucleosomal DNA and is associated with transcriptionally active chromatin. Along with a similar protein, HMGN1, the encoded protein may help maintain an open chromatin configuration around transcribable genes. The protein has also been found to have antimicrobial activity against bacteria, viruses and fungi.
Genomic regions Chromosome 1
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
HMGN2-201 ENST00000361427.6 1940 90aa Protein coding CCDS283 P05204 NM_005517.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
HMGN2-210 ENST00000619352.4 1148 90aa Protein coding CCDS283 P05204 - TSL:1, GENCODE basic, APPRIS P1,
HMGN2-209 ENST00000493418.1 1496 No protein Processed transcript - - - TSL:2,
HMGN2-202 ENST00000460563.5 1045 No protein Processed transcript - - - TSL:5,
HMGN2-208 ENST00000479815.5 1030 No protein Processed transcript - - - TSL:5,
HMGN2-205 ENST00000466194.1 952 No protein Processed transcript - - - TSL:2,
HMGN2-206 ENST00000467700.5 932 No protein Processed transcript - - - TSL:2,
HMGN2-203 ENST00000463817.5 853 No protein Processed transcript - - - TSL:2,
HMGN2-204 ENST00000464888.5 565 No protein Processed transcript - - - TSL:2,
HMGN2-207 ENST00000468388.5 320 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene HMGN2
This KO Strategy loading
Red Cotton™ Notes Gene HMGN2 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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