CRISPR-U™ Gene Knockout Cell Line
Strategy
HMGB2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human HMGB2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | HMGB2 |
| Gene id | 3148 |
| Organism | Homo sapiens |
| Official full symbol | high mobility group box 2 |
| Gene type | protein-coding |
| Also known as | HMG2 |
| Summary | This gene encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination. |
| Genomic regions | Chromosome 4 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| HMGB2-201 | ENST00000296503.10 | 1441 | 209aa | Protein coding | CCDS3816 | P26583 | NM_002129.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| HMGB2-203 | ENST00000446922.6 | 1175 | 209aa | Protein coding | CCDS3816 | P26583 | - | TSL:1, GENCODE basic, APPRIS P1, |
| HMGB2-202 | ENST00000438704.6 | 1032 | 209aa | Protein coding | CCDS3816 | P26583 | - | TSL:1, GENCODE basic, APPRIS P1, |
| HMGB2-204 | ENST00000506267.1 | 629 | 134aa | Protein coding | - | D6R9A6 | - | CDS 3' incomplete, TSL:3, |
| HMGB2-205 | ENST00000511316.1 | 1841 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | HMGB2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene HMGB2 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: