CRISPR-U™ Gene Knockout Cell Line Strategy

HMGB2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human HMGB2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol HMGB2
Gene id 3148
Organism Homo sapiens
Official full symbol high mobility group box 2
Gene type protein-coding
Also known as HMG2
Summary This gene encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination.
Genomic regions Chromosome 4
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
HMGB2-201 ENST00000296503.10 1441 209aa Protein coding CCDS3816 P26583 NM_002129.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
HMGB2-203 ENST00000446922.6 1175 209aa Protein coding CCDS3816 P26583 - TSL:1, GENCODE basic, APPRIS P1,
HMGB2-202 ENST00000438704.6 1032 209aa Protein coding CCDS3816 P26583 - TSL:1, GENCODE basic, APPRIS P1,
HMGB2-204 ENST00000506267.1 629 134aa Protein coding - D6R9A6 - CDS 3' incomplete, TSL:3,
HMGB2-205 ENST00000511316.1 1841 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene HMGB2
This KO Strategy loading
Red Cotton™ Notes Gene HMGB2 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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