CRISPR-U™ Gene Knockout Cell Line Strategy

HMGB1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human HMGB1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol HMGB1
Gene id 3146
Organism Homo sapiens
Official full symbol high mobility group box 1
Gene type protein-coding
Also known as HMG-1, HMG1, HMG3, SBP-1
Summary This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein.
Genomic regions Chromosome 13
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
HMGB1-203 ENST00000341423.10 5456 215aa Protein coding CCDS9335 A0A024RDR0 P09429 NM_002128.7 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
HMGB1-206 ENST00000405805.5 4199 215aa Protein coding CCDS9335 A0A024RDR0 P09429 - TSL:2, GENCODE basic, APPRIS P1,
HMGB1-202 ENST00000339872.8 2319 215aa Protein coding CCDS9335 A0A024RDR0 P09429 - TSL:2, GENCODE basic, APPRIS P1,
HMGB1-204 ENST00000399489.5 1727 158aa Protein coding CCDS86347 Q5T7C4 - TSL:1, GENCODE basic,
HMGB1-205 ENST00000399494.5 1282 215aa Protein coding CCDS9335 A0A024RDR0 P09429 - TSL:2, GENCODE basic, APPRIS P1,
HMGB1-201 ENST00000326004.4 821 158aa Protein coding CCDS86347 Q5T7C4 - TSL:3, GENCODE basic,
HMGB1-207 ENST00000468384.1 503 No protein Processed transcript - - - TSL:1,
HMGB1-208 ENST00000490788.1 425 No protein Processed transcript - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene HMGB1
This KO Strategy loading
Red Cotton™ Notes Gene HMGB1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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