CRISPR-U™ Gene Knockout Cell Line
Strategy
HMGB1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human HMGB1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | HMGB1 |
| Gene id | 3146 |
| Organism | Homo sapiens |
| Official full symbol | high mobility group box 1 |
| Gene type | protein-coding |
| Also known as | HMG-1, HMG1, HMG3, SBP-1 |
| Summary | This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. |
| Genomic regions | Chromosome 13 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| HMGB1-203 | ENST00000341423.10 | 5456 | 215aa | Protein coding | CCDS9335 | A0A024RDR0 P09429 | NM_002128.7 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| HMGB1-206 | ENST00000405805.5 | 4199 | 215aa | Protein coding | CCDS9335 | A0A024RDR0 P09429 | - | TSL:2, GENCODE basic, APPRIS P1, |
| HMGB1-202 | ENST00000339872.8 | 2319 | 215aa | Protein coding | CCDS9335 | A0A024RDR0 P09429 | - | TSL:2, GENCODE basic, APPRIS P1, |
| HMGB1-204 | ENST00000399489.5 | 1727 | 158aa | Protein coding | CCDS86347 | Q5T7C4 | - | TSL:1, GENCODE basic, |
| HMGB1-205 | ENST00000399494.5 | 1282 | 215aa | Protein coding | CCDS9335 | A0A024RDR0 P09429 | - | TSL:2, GENCODE basic, APPRIS P1, |
| HMGB1-201 | ENST00000326004.4 | 821 | 158aa | Protein coding | CCDS86347 | Q5T7C4 | - | TSL:3, GENCODE basic, |
| HMGB1-207 | ENST00000468384.1 | 503 | No protein | Processed transcript | - | - | - | TSL:1, |
| HMGB1-208 | ENST00000490788.1 | 425 | No protein | Processed transcript | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | HMGB1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene HMGB1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$39
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: