CRISPR-U™ Gene Knockout Cell Line
Strategy
NRG1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NRG1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NRG1 |
| Gene id | 3084 |
| Organism | Homo sapiens |
| Official full symbol | neuregulin 1 |
| Gene type | protein-coding |
| Also known as | ARIA, GGF, GGF2, HGL, HRG, HRG1, HRGA, MST131, MSTP131, NDF, NRG1-IT2, SMDF |
| Summary | The protein encoded by this gene is a membrane glycoprotein that mediates cell-cell signaling and plays a critical role in the growth and development of multiple organ systems. An extraordinary variety of different isoforms are produced from this gene through alternative promoter usage and splicing. These isoforms are expressed in a tissue-specific manner and differ significantly in their structure, and are classified as types I, II, III, IV, V and VI. Dysregulation of this gene has been linked to diseases such as cancer, schizophrenia, and bipolar disorder (BPD). |
| Genomic regions | Chromosome 8 |
Strategy Summary
This gene has 28 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NRG1-202 | ENST00000356819.7 | 5931 | 637aa | Protein coding | CCDS6084 | Q02297-7 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| NRG1-219 | ENST00000539990.3 | 2401 | 308aa | Protein coding | CCDS75727 | F6RJN6 | - | TSL:2, GENCODE basic, |
| NRG1-215 | ENST00000523079.5 | 2359 | 420aa | Protein coding | CCDS55219 | Q02297-12 | - | TSL:2, GENCODE basic, |
| NRG1-201 | ENST00000287842.7 | 2035 | 645aa | Protein coding | CCDS6083 | Q02297-6 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| NRG1-203 | ENST00000405005.7 | 1992 | 640aa | Protein coding | CCDS6085 | Q02297-1 | - | TSL:1, GENCODE basic, APPRIS P4, |
| NRG1-209 | ENST00000520407.5 | 1955 | 422aa | Protein coding | CCDS47836 | Q02297-9 | - | TSL:1, GENCODE basic, |
| NRG1-208 | ENST00000519301.6 | 1949 | 590aa | Protein coding | CCDS55218 | Q02297-11 | - | TSL:5, GENCODE basic, |
| NRG1-210 | ENST00000520502.7 | 1945 | 296aa | Protein coding | CCDS6087 | Q02297-10 | - | TSL:1, GENCODE basic, |
| NRG1-211 | ENST00000521670.5 | 1769 | 462aa | Protein coding | CCDS6086 | Q02297-3 | - | TSL:1, GENCODE basic, |
| NRG1-237 | ENST00000652698.1 | 11618 | 607aa | Protein coding | - | A0A494C1F8 | - | GENCODE basic, APPRIS ALT2, |
| NRG1-236 | ENST00000652592.1 | 6346 | 700aa | Protein coding | - | A0A494C0Q4 | - | GENCODE basic, |
| NRG1-222 | ENST00000650819.1 | 3664 | 483aa | Protein coding | - | A0A494C1B5 | - | GENCODE basic, |
| NRG1-231 | ENST00000651333.1 | 3021 | 522aa | Protein coding | - | A0A494C0L9 | - | GENCODE basic, |
| NRG1-217 | ENST00000523534.5 | 2516 | 713aa | Protein coding | - | H0YBA3 | - | CDS 5' incomplete, TSL:5, |
| NRG1-232 | ENST00000651335.1 | 2281 | 387aa | Protein coding | - | A0A494C1G2 | - | CDS 5' incomplete, |
| NRG1-228 | ENST00000650980.1 | 2238 | 459aa | Protein coding | - | A0A494C0K4 | - | GENCODE basic, |
| NRG1-224 | ENST00000650866.1 | 1966 | 624aa | Protein coding | - | A0A494C1F5 | - | CDS 3' incomplete, |
| NRG1-235 | ENST00000652588.1 | 1926 | 461aa | Protein coding | - | A0A494C043 | - | GENCODE basic, |
| NRG1-214 | ENST00000523041.2 | 1829 | 236aa | Protein coding | - | H0YDC2 | - | TSL:5, GENCODE basic, |
| NRG1-225 | ENST00000650919.1 | 1703 | 241aa | Protein coding | - | Q02297-8 | - | GENCODE basic, |
| NRG1-227 | ENST00000650967.1 | 1480 | 207aa | Protein coding | - | A0A494C114 | - | GENCODE basic, |
| NRG1-206 | ENST00000518206.5 | 886 | 126aa | Protein coding | - | H0YCP0 | - | CDS 5' incomplete, TSL:1, |
| NRG1-220 | ENST00000614767.1 | 762 | 254aa | Protein coding | - | A0A087X0X9 | - | TSL:5, GENCODE basic, |
| NRG1-213 | ENST00000522569.1 | 659 | 168aa | Protein coding | - | A0A5F9ZHA5 | - | CDS 3' incomplete, TSL:3, |
| NRG1-212 | ENST00000522402.5 | 570 | 128aa | Protein coding | - | E5RHP6 | - | CDS 3' incomplete, TSL:4, |
| NRG1-207 | ENST00000519240.5 | 563 | 135aa | Protein coding | - | E5RIG8 | - | CDS 3' incomplete, TSL:4, |
| NRG1-229 | ENST00000651149.1 | 557 | 155aa | Protein coding | - | A0A494C054 | - | CDS 3' incomplete, |
| NRG1-205 | ENST00000518084.5 | 555 | 101aa | Protein coding | - | E5RHQ1 | - | CDS 3' incomplete, TSL:3, |
| NRG1-233 | ENST00000651696.1 | 5183 | 130aa | Nonsense mediated decay | - | A0A494C0T5 | - | CDS 5' incomplete, |
| NRG1-223 | ENST00000650856.1 | 1832 | 214aa | Nonsense mediated decay | - | A0A494BZT4 | - | - |
| NRG1-218 | ENST00000523681.1 | 1173 | No protein | Processed transcript | - | - | - | TSL:4, |
| NRG1-221 | ENST00000631040.2 | 623 | No protein | Processed transcript | - | - | - | TSL:5, |
| NRG1-216 | ENST00000523320.1 | 553 | No protein | Processed transcript | - | - | - | TSL:4, |
| NRG1-204 | ENST00000517967.1 | 543 | No protein | Processed transcript | - | - | - | TSL:3, |
| NRG1-234 | ENST00000651807.1 | 1614 | No protein | Retained intron | - | - | - | - |
| NRG1-230 | ENST00000651175.1 | 590 | No protein | Retained intron | - | - | - | - |
| NRG1-226 | ENST00000650964.1 | 543 | No protein | Retained intron | - | - | - | - |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NRG1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NRG1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
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Work flow
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