CRISPR-U™ Gene Knockout Cell Line Strategy

NRG1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human NRG1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NRG1
Gene id 3084
Organism Homo sapiens
Official full symbol neuregulin 1
Gene type protein-coding
Also known as ARIA, GGF, GGF2, HGL, HRG, HRG1, HRGA, MST131, MSTP131, NDF, NRG1-IT2, SMDF
Summary The protein encoded by this gene is a membrane glycoprotein that mediates cell-cell signaling and plays a critical role in the growth and development of multiple organ systems. An extraordinary variety of different isoforms are produced from this gene through alternative promoter usage and splicing. These isoforms are expressed in a tissue-specific manner and differ significantly in their structure, and are classified as types I, II, III, IV, V and VI. Dysregulation of this gene has been linked to diseases such as cancer, schizophrenia, and bipolar disorder (BPD).
Genomic regions Chromosome 8
Strategy Summary
This gene has 28 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NRG1-202 ENST00000356819.7 5931 637aa Protein coding CCDS6084 Q02297-7 - TSL:1, GENCODE basic, APPRIS ALT2,
NRG1-219 ENST00000539990.3 2401 308aa Protein coding CCDS75727 F6RJN6 - TSL:2, GENCODE basic,
NRG1-215 ENST00000523079.5 2359 420aa Protein coding CCDS55219 Q02297-12 - TSL:2, GENCODE basic,
NRG1-201 ENST00000287842.7 2035 645aa Protein coding CCDS6083 Q02297-6 - TSL:1, GENCODE basic, APPRIS ALT2,
NRG1-203 ENST00000405005.7 1992 640aa Protein coding CCDS6085 Q02297-1 - TSL:1, GENCODE basic, APPRIS P4,
NRG1-209 ENST00000520407.5 1955 422aa Protein coding CCDS47836 Q02297-9 - TSL:1, GENCODE basic,
NRG1-208 ENST00000519301.6 1949 590aa Protein coding CCDS55218 Q02297-11 - TSL:5, GENCODE basic,
NRG1-210 ENST00000520502.7 1945 296aa Protein coding CCDS6087 Q02297-10 - TSL:1, GENCODE basic,
NRG1-211 ENST00000521670.5 1769 462aa Protein coding CCDS6086 Q02297-3 - TSL:1, GENCODE basic,
NRG1-237 ENST00000652698.1 11618 607aa Protein coding - A0A494C1F8 - GENCODE basic, APPRIS ALT2,
NRG1-236 ENST00000652592.1 6346 700aa Protein coding - A0A494C0Q4 - GENCODE basic,
NRG1-222 ENST00000650819.1 3664 483aa Protein coding - A0A494C1B5 - GENCODE basic,
NRG1-231 ENST00000651333.1 3021 522aa Protein coding - A0A494C0L9 - GENCODE basic,
NRG1-217 ENST00000523534.5 2516 713aa Protein coding - H0YBA3 - CDS 5' incomplete, TSL:5,
NRG1-232 ENST00000651335.1 2281 387aa Protein coding - A0A494C1G2 - CDS 5' incomplete,
NRG1-228 ENST00000650980.1 2238 459aa Protein coding - A0A494C0K4 - GENCODE basic,
NRG1-224 ENST00000650866.1 1966 624aa Protein coding - A0A494C1F5 - CDS 3' incomplete,
NRG1-235 ENST00000652588.1 1926 461aa Protein coding - A0A494C043 - GENCODE basic,
NRG1-214 ENST00000523041.2 1829 236aa Protein coding - H0YDC2 - TSL:5, GENCODE basic,
NRG1-225 ENST00000650919.1 1703 241aa Protein coding - Q02297-8 - GENCODE basic,
NRG1-227 ENST00000650967.1 1480 207aa Protein coding - A0A494C114 - GENCODE basic,
NRG1-206 ENST00000518206.5 886 126aa Protein coding - H0YCP0 - CDS 5' incomplete, TSL:1,
NRG1-220 ENST00000614767.1 762 254aa Protein coding - A0A087X0X9 - TSL:5, GENCODE basic,
NRG1-213 ENST00000522569.1 659 168aa Protein coding - A0A5F9ZHA5 - CDS 3' incomplete, TSL:3,
NRG1-212 ENST00000522402.5 570 128aa Protein coding - E5RHP6 - CDS 3' incomplete, TSL:4,
NRG1-207 ENST00000519240.5 563 135aa Protein coding - E5RIG8 - CDS 3' incomplete, TSL:4,
NRG1-229 ENST00000651149.1 557 155aa Protein coding - A0A494C054 - CDS 3' incomplete,
NRG1-205 ENST00000518084.5 555 101aa Protein coding - E5RHQ1 - CDS 3' incomplete, TSL:3,
NRG1-233 ENST00000651696.1 5183 130aa Nonsense mediated decay - A0A494C0T5 - CDS 5' incomplete,
NRG1-223 ENST00000650856.1 1832 214aa Nonsense mediated decay - A0A494BZT4 - -
NRG1-218 ENST00000523681.1 1173 No protein Processed transcript - - - TSL:4,
NRG1-221 ENST00000631040.2 623 No protein Processed transcript - - - TSL:5,
NRG1-216 ENST00000523320.1 553 No protein Processed transcript - - - TSL:4,
NRG1-204 ENST00000517967.1 543 No protein Processed transcript - - - TSL:3,
NRG1-234 ENST00000651807.1 1614 No protein Retained intron - - - -
NRG1-230 ENST00000651175.1 590 No protein Retained intron - - - -
NRG1-226 ENST00000650964.1 543 No protein Retained intron - - - -
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NRG1
This KO Strategy loading
Red Cotton™ Notes Gene NRG1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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