CRISPR-U™ Gene Knockout Cell Line Strategy

HELLS Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human HELLS Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol HELLS
Gene id 3070
Organism Homo sapiens
Official full symbol helicase, lymphoid specific
Gene type protein-coding
Also known as ICF4, LSH, Nbla10143, PASG, SMARCA6
Summary This gene encodes a lymphoid-specific helicase. Other helicases function in processes involving DNA strand separation, including replication, repair, recombination, and transcription. This protein is thought to be involved with cellular proliferation and may play a role in leukemogenesis. Alternatively spliced transcript variants encoding different isoforms have been identified.
Genomic regions Chromosome 10
Strategy Summary
This gene has 8 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
HELLS-205 ENST00000394036.5 3131 884aa Protein coding CCDS73162 A0A0B4J1V9 - TSL:1, GENCODE basic,
HELLS-202 ENST00000348459.10 3128 838aa Protein coding CCDS7434 Q9NRZ9-1 NM_018063.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
HELLS-201 ENST00000239026.10 2740 714aa Protein coding CCDS73165 F6XU50 - TSL:5, GENCODE basic,
HELLS-206 ENST00000394045.5 2699 740aa Protein coding CCDS73163 Q9NRZ9-5 - TSL:1, GENCODE basic,
HELLS-204 ENST00000371332.8 2675 700aa Protein coding CCDS73164 A0A087WSW7 - TSL:5, GENCODE basic,
HELLS-203 ENST00000371327.2 4987 310aa Protein coding - Q9NW36 - CDS 5' incomplete, TSL:2,
HELLS-207 ENST00000419900.5 711 192aa Protein coding - B1ALG6 - CDS 3' incomplete, TSL:3,
HELLS-212 ENST00000630929.2 400 97aa Protein coding - Q76H82 - TSL:5, GENCODE basic,
HELLS-208 ENST00000462057.2 778 No protein Processed transcript - - - TSL:3,
HELLS-210 ENST00000466552.1 2724 No protein Retained intron - - - TSL:2,
HELLS-211 ENST00000475263.1 676 No protein Retained intron - - - TSL:3,
HELLS-209 ENST00000464030.1 321 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene HELLS
This KO Strategy loading
Red Cotton™ Notes Gene HELLS had been KO in hct116 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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