CRISPR-U™ Gene Knockout Cell Line Strategy

HAGH Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human HAGH Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol HAGH
Gene id 3029
Organism Homo sapiens
Official full symbol hydroxyacylglutathione hydrolase
Gene type protein-coding
Also known as GLO2, GLX2, GLXII, HAGH1
Summary The enzyme encoded by this gene is classified as a thiolesterase and is responsible for the hydrolysis of S-lactoyl-glutathione to reduced glutathione and D-lactate. Three transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 16
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
HAGH-202 ENST00000397356.8 2619 308aa Protein coding CCDS10447 Q16775-1 NM_005326.6 TSL:1, GENCODE basic, MANE Select v0.92,
HAGH-208 ENST00000566709.5 1291 258aa Protein coding CCDS86494 H3BPQ4 - TSL:5, GENCODE basic,
HAGH-203 ENST00000455446.6 1276 236aa Protein coding CCDS66900 Q16775-3 - TSL:2, GENCODE basic,
HAGH-201 ENST00000397353.6 1257 260aa Protein coding CCDS32366 Q16775-2 - TSL:5, GENCODE basic, APPRIS P1,
HAGH-204 ENST00000564445.5 864 239aa Protein coding - H3BPK3 - CDS 5' incomplete, TSL:3,
HAGH-211 ENST00000569339.1 820 160aa Protein coding - H3BQW8 - CDS 3' incomplete, TSL:3,
HAGH-207 ENST00000566644.5 612 64aa Protein coding - H3BV79 - CDS 5' incomplete, TSL:5,
HAGH-210 ENST00000567398.1 1473 No protein Processed transcript - - - TSL:2,
HAGH-212 ENST00000569700.5 1156 No protein Retained intron - - - TSL:1,
HAGH-206 ENST00000565097.1 1147 No protein Retained intron - - - TSL:2,
HAGH-205 ENST00000564518.5 513 No protein Retained intron - - - TSL:2,
HAGH-209 ENST00000567190.5 472 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene HAGH
This KO Strategy loading
Red Cotton™ Notes Gene HAGH had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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