CRISPR-U™ Gene Knockout Cell Line
Strategy
HAGH Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human HAGH Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | HAGH |
| Gene id | 3029 |
| Organism | Homo sapiens |
| Official full symbol | hydroxyacylglutathione hydrolase |
| Gene type | protein-coding |
| Also known as | GLO2, GLX2, GLXII, HAGH1 |
| Summary | The enzyme encoded by this gene is classified as a thiolesterase and is responsible for the hydrolysis of S-lactoyl-glutathione to reduced glutathione and D-lactate. Three transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 16 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| HAGH-202 | ENST00000397356.8 | 2619 | 308aa | Protein coding | CCDS10447 | Q16775-1 | NM_005326.6 | TSL:1, GENCODE basic, MANE Select v0.92, |
| HAGH-208 | ENST00000566709.5 | 1291 | 258aa | Protein coding | CCDS86494 | H3BPQ4 | - | TSL:5, GENCODE basic, |
| HAGH-203 | ENST00000455446.6 | 1276 | 236aa | Protein coding | CCDS66900 | Q16775-3 | - | TSL:2, GENCODE basic, |
| HAGH-201 | ENST00000397353.6 | 1257 | 260aa | Protein coding | CCDS32366 | Q16775-2 | - | TSL:5, GENCODE basic, APPRIS P1, |
| HAGH-204 | ENST00000564445.5 | 864 | 239aa | Protein coding | - | H3BPK3 | - | CDS 5' incomplete, TSL:3, |
| HAGH-211 | ENST00000569339.1 | 820 | 160aa | Protein coding | - | H3BQW8 | - | CDS 3' incomplete, TSL:3, |
| HAGH-207 | ENST00000566644.5 | 612 | 64aa | Protein coding | - | H3BV79 | - | CDS 5' incomplete, TSL:5, |
| HAGH-210 | ENST00000567398.1 | 1473 | No protein | Processed transcript | - | - | - | TSL:2, |
| HAGH-212 | ENST00000569700.5 | 1156 | No protein | Retained intron | - | - | - | TSL:1, |
| HAGH-206 | ENST00000565097.1 | 1147 | No protein | Retained intron | - | - | - | TSL:2, |
| HAGH-205 | ENST00000564518.5 | 513 | No protein | Retained intron | - | - | - | TSL:2, |
| HAGH-209 | ENST00000567190.5 | 472 | No protein | Retained intron | - | - | - | TSL:4, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | HAGH |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene HAGH had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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