CRISPR-U™ Gene Knockout Cell Line Strategy

NRBF2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NRBF2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NRBF2
Gene id 29982
Organism Homo sapiens
Official full symbol nuclear receptor binding factor 2
Gene type protein-coding
Also known as COPR, COPR1, COPR2, NRBF-2
Genomic regions Chromosome 10
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NRBF2-202 ENST00000435510.6 1816 277aa Protein coding CCDS60537 Q96F24-3 - TSL:2, GENCODE basic,
NRBF2-201 ENST00000277746.11 1813 287aa Protein coding CCDS7268 Q96F24-1 NM_030759.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NRBF2
This KO Strategy loading
Red Cotton™ Notes Gene NRBF2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$49

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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