CRISPR-U™ Gene Knockout Cell Line Strategy

GYS1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human GYS1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol GYS1
Gene id 2997
Organism Homo sapiens
Official full symbol glycogen synthase 1
Gene type protein-coding
Also known as GSY, GYS
Summary The protein encoded by this gene catalyzes the addition of glucose monomers to the growing glycogen molecule through the formation of alpha-1,4-glycoside linkages. Mutations in this gene are associated with muscle glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 19
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
GYS1-202 ENST00000323798.8 3569 737aa Protein coding CCDS12747 P13807-1 NM_002103.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
GYS1-201 ENST00000263276.6 3378 673aa Protein coding CCDS54292 P13807-2 - TSL:1, GENCODE basic,
GYS1-207 ENST00000594220.1 427 113aa Protein coding - M0QYU1 - CDS 5' incomplete, TSL:NA,
GYS1-203 ENST00000457974.1 718 No protein Processed transcript - - - TSL:3,
GYS1-204 ENST00000472004.5 578 No protein Retained intron - - - TSL:3,
GYS1-206 ENST00000496048.1 536 No protein Retained intron - - - TSL:3,
GYS1-205 ENST00000484289.2 459 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene GYS1
This KO Strategy loading
Red Cotton™ Notes Gene GYS1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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