CRISPR-U™ Gene Knockout Cell Line
Strategy
PSAT1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PSAT1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PSAT1 |
| Gene id | 29968 |
| Organism | Homo sapiens |
| Official full symbol | phosphoserine aminotransferase 1 |
| Gene type | protein-coding |
| Also known as | EPIP, NLS2, PSA, PSAT, PSATD |
| Summary | This gene encodes a member of the class-V pyridoxal-phosphate-dependent aminotransferase family. The encoded protein is a phosphoserine aminotransferase and decreased expression may be associated with schizophrenia. Mutations in this gene are also associated with phosphoserine aminotransferase deficiency. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 1, 3, and 8. |
| Genomic regions | Chromosome 9 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PSAT1-202 | ENST00000376588.4 | 2206 | 370aa | Protein coding | CCDS6660 | Q9Y617-1 | NM_058179.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PSAT1-201 | ENST00000347159.6 | 1411 | 324aa | Protein coding | CCDS6659 | Q9Y617-2 | - | TSL:1, GENCODE basic, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PSAT1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PSAT1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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