CRISPR-U™ Gene Knockout Cell Line Strategy

GPSM2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human GPSM2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol GPSM2
Gene id 29899
Organism Homo sapiens
Official full symbol G protein signaling modulator 2
Gene type protein-coding
Also known as CMCS, DFNB82, LGN, PINS
Summary The protein encoded by this gene belongs to a family of proteins that modulate activation of G proteins, which transduce extracellular signals received by cell surface receptors into integrated cellular responses. The N-terminal half of this protein contains 10 copies of leu-gly-asn (LGN) repeat, and the C-terminal half contains 4 GoLoco motifs, which are involved in guanine nucleotide exchange. This protein may play a role in neuroblast division and in the development of normal hearing. Mutations in this gene are associated with autosomal recessive nonsyndromic deafness (DFNB82). Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 1
Strategy Summary
This gene has 16 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
GPSM2-202 ENST00000406462.6 7433 684aa Protein coding CCDS792 A0A024R0F8 P81274 - TSL:5, GENCODE basic, APPRIS P1,
GPSM2-201 ENST00000264126.9 7152 684aa Protein coding CCDS792 A0A024R0F8 P81274 NM_013296.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
GPSM2-207 ENST00000642355.1 5609 684aa Protein coding CCDS792 A0A024R0F8 P81274 - GENCODE basic, APPRIS P1,
GPSM2-206 ENST00000446797.2 3194 684aa Protein coding CCDS792 A0A024R0F8 B0QZC9 - TSL:4, GENCODE basic, APPRIS P1,
GPSM2-211 ENST00000645164.2 2806 684aa Protein coding CCDS792 A0A024R0F8 A0A2R8Y896 - GENCODE basic, APPRIS P1,
GPSM2-223 ENST00000676184.1 2741 684aa Protein coding CCDS792 A0A024R0F8 - GENCODE basic, APPRIS P1,
GPSM2-217 ENST00000675086.1 2991 625aa Protein coding - - - GENCODE basic,
GPSM2-218 ENST00000675087.1 2812 701aa Protein coding - - - GENCODE basic,
GPSM2-216 ENST00000674914.1 2700 701aa Protein coding - - - GENCODE basic,
GPSM2-214 ENST00000674700.1 2447 519aa Protein coding - - - GENCODE basic,
GPSM2-205 ENST00000441735.2 2378 627aa Protein coding - H0Y4A4 - TSL:2, GENCODE basic,
GPSM2-209 ENST00000643643.1 1520 311aa Protein coding - A0A2R8Y6E3 - CDS 5' incomplete,
GPSM2-203 ENST00000435475.5 992 75aa Protein coding - B0QZD0 - CDS 3' incomplete, TSL:3,
GPSM2-204 ENST00000435987.5 904 213aa Protein coding - Q5T1N9 - CDS 3' incomplete, TSL:3,
GPSM2-208 ENST00000643094.1 799 93aa Protein coding - A0A2R8Y673 - CDS 3' incomplete,
GPSM2-212 ENST00000645255.1 685 228aa Protein coding - A0A2R8YCX1 - CDS 5' and 3' incomplete,
GPSM2-215 ENST00000674731.1 3841 384aa Nonsense mediated decay - - - -
GPSM2-224 ENST00000676404.1 2472 465aa Nonsense mediated decay - - - -
GPSM2-219 ENST00000675617.1 2386 No protein Processed transcript - - - -
GPSM2-213 ENST00000674663.1 1549 No protein Processed transcript - - - -
GPSM2-210 ENST00000643921.1 563 No protein Processed transcript - - - -
GPSM2-221 ENST00000675776.1 8071 No protein Retained intron - - - -
GPSM2-222 ENST00000675829.1 3284 No protein Retained intron - - - -
GPSM2-220 ENST00000675740.1 2107 No protein Retained intron - - - -
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene GPSM2
This KO Strategy loading
Red Cotton™ Notes Gene GPSM2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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