CRISPR-U™ Gene Knockout Cell Line Strategy

MSH6 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MSH6 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MSH6
Gene id 2956
Organism Homo sapiens
Official full symbol mutS homolog 6
Gene type protein-coding
Also known as GTBP, GTMBP, HNPCC5, HSAP, p160
Summary This gene encodes a member of the DNA mismatch repair MutS family. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein heterodimerizes with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene may be associated with hereditary nonpolyposis colon cancer, colorectal cancer, and endometrial cancer. Transcripts variants encoding different isoforms have been described.
Genomic regions Chromosome 2
Strategy Summary
This gene has 11 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MSH6-201 ENST00000234420.11 4265 1360aa Protein coding CCDS1836 P52701-1 NM_000179.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
MSH6-212 ENST00000614496.4 4150 1058aa Protein coding CCDS62907 P52701-4 - TSL:1, GENCODE basic,
MSH6-209 ENST00000538136.1 4055 1058aa Protein coding CCDS62907 P52701-4 - TSL:2, GENCODE basic,
MSH6-210 ENST00000540021.6 3829 1230aa Protein coding CCDS62906 P52701-3 - TSL:2, GENCODE basic,
MSH6-215 ENST00000652107.1 7669 1261aa Protein coding - A0A494C0M1 - GENCODE basic,
MSH6-214 ENST00000622629.4 4324 327aa Protein coding - A0A087WYT6 - TSL:5, GENCODE basic,
MSH6-216 ENST00000673637.1 3959 1261aa Protein coding - A0A494C0M1 - GENCODE basic,
MSH6-213 ENST00000616033.4 3429 1067aa Protein coding - A0A087WWJ1 - TSL:5, GENCODE basic,
MSH6-206 ENST00000455383.5 578 115aa Protein coding - C9J8Y8 - CDS 3' incomplete, TSL:5,
MSH6-202 ENST00000411819.1 548 151aa Protein coding - C9J7Y7 - CDS 3' incomplete, TSL:4,
MSH6-203 ENST00000420813.5 491 101aa Protein coding - C9JH55 - CDS 3' incomplete, TSL:4,
MSH6-204 ENST00000445503.5 4158 160aa Nonsense mediated decay - F8WAX8 - TSL:1,
MSH6-207 ENST00000456246.1 846 104aa Nonsense mediated decay - F8W7G9 - TSL:3,
MSH6-211 ENST00000607272.2 2219 No protein Processed transcript - - - TSL:2,
MSH6-208 ENST00000493177.1 813 No protein Processed transcript - - - TSL:3,
MSH6-205 ENST00000454137.5 725 No protein Processed transcript - - - TSL:3,
MSH6-217 ENST00000673922.1 573 No protein Processed transcript - - - -
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MSH6
This KO Strategy loading
Red Cotton™ Notes Gene MSH6 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

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