CRISPR-U™ Gene Knockout Cell Line
Strategy
MSH6 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MSH6 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MSH6 |
| Gene id | 2956 |
| Organism | Homo sapiens |
| Official full symbol | mutS homolog 6 |
| Gene type | protein-coding |
| Also known as | GTBP, GTMBP, HNPCC5, HSAP, p160 |
| Summary | This gene encodes a member of the DNA mismatch repair MutS family. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein heterodimerizes with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene may be associated with hereditary nonpolyposis colon cancer, colorectal cancer, and endometrial cancer. Transcripts variants encoding different isoforms have been described. |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 11 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MSH6-201 | ENST00000234420.11 | 4265 | 1360aa | Protein coding | CCDS1836 | P52701-1 | NM_000179.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| MSH6-212 | ENST00000614496.4 | 4150 | 1058aa | Protein coding | CCDS62907 | P52701-4 | - | TSL:1, GENCODE basic, |
| MSH6-209 | ENST00000538136.1 | 4055 | 1058aa | Protein coding | CCDS62907 | P52701-4 | - | TSL:2, GENCODE basic, |
| MSH6-210 | ENST00000540021.6 | 3829 | 1230aa | Protein coding | CCDS62906 | P52701-3 | - | TSL:2, GENCODE basic, |
| MSH6-215 | ENST00000652107.1 | 7669 | 1261aa | Protein coding | - | A0A494C0M1 | - | GENCODE basic, |
| MSH6-214 | ENST00000622629.4 | 4324 | 327aa | Protein coding | - | A0A087WYT6 | - | TSL:5, GENCODE basic, |
| MSH6-216 | ENST00000673637.1 | 3959 | 1261aa | Protein coding | - | A0A494C0M1 | - | GENCODE basic, |
| MSH6-213 | ENST00000616033.4 | 3429 | 1067aa | Protein coding | - | A0A087WWJ1 | - | TSL:5, GENCODE basic, |
| MSH6-206 | ENST00000455383.5 | 578 | 115aa | Protein coding | - | C9J8Y8 | - | CDS 3' incomplete, TSL:5, |
| MSH6-202 | ENST00000411819.1 | 548 | 151aa | Protein coding | - | C9J7Y7 | - | CDS 3' incomplete, TSL:4, |
| MSH6-203 | ENST00000420813.5 | 491 | 101aa | Protein coding | - | C9JH55 | - | CDS 3' incomplete, TSL:4, |
| MSH6-204 | ENST00000445503.5 | 4158 | 160aa | Nonsense mediated decay | - | F8WAX8 | - | TSL:1, |
| MSH6-207 | ENST00000456246.1 | 846 | 104aa | Nonsense mediated decay | - | F8W7G9 | - | TSL:3, |
| MSH6-211 | ENST00000607272.2 | 2219 | No protein | Processed transcript | - | - | - | TSL:2, |
| MSH6-208 | ENST00000493177.1 | 813 | No protein | Processed transcript | - | - | - | TSL:3, |
| MSH6-205 | ENST00000454137.5 | 725 | No protein | Processed transcript | - | - | - | TSL:3, |
| MSH6-217 | ENST00000673922.1 | 573 | No protein | Processed transcript | - | - | - | - |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MSH6 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MSH6 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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