CRISPR-U™ Gene Knockout Cell Line
Strategy
GSS Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human GSS Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | GSS |
| Gene id | 2937 |
| Organism | Homo sapiens |
| Official full symbol | glutathione synthetase |
| Gene type | protein-coding |
| Also known as | GSHS, HEL-S-64p, HEL-S-88n |
| Summary | Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency. |
| Genomic regions | Chromosome 20 |
Strategy Summary
This gene has 11 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| GSS-217 | ENST00000644793.1 | 2702 | 474aa | Protein coding | CCDS13245 | P48637-1 | - | GENCODE basic, APPRIS P1, |
| GSS-205 | ENST00000643188.1 | 1940 | 474aa | Protein coding | CCDS13245 | P48637-1 | - | GENCODE basic, APPRIS P1, |
| GSS-228 | ENST00000651619.1 | 1909 | 474aa | Protein coding | CCDS13245 | P48637-1 | NM_000178.4 | GENCODE basic, APPRIS P1, MANE Select v0.92, |
| GSS-203 | ENST00000642498.1 | 2131 | 450aa | Protein coding | - | A0A2R8Y430 | - | GENCODE basic, |
| GSS-226 | ENST00000646735.1 | 1554 | 363aa | Protein coding | - | P48637-2 | - | GENCODE basic, |
| GSS-209 | ENST00000643502.1 | 1310 | 360aa | Protein coding | - | A0A2R8Y5T7 | - | CDS 5' incomplete, |
| GSS-201 | ENST00000451957.2 | 1092 | 363aa | Protein coding | - | P48637-2 | - | TSL:1, GENCODE basic, |
| GSS-219 | ENST00000645328.1 | 870 | 169aa | Protein coding | - | A0A2R8Y6Q7 | - | CDS 5' incomplete, |
| GSS-207 | ENST00000643271.1 | 587 | 134aa | Protein coding | - | A0A2R8YF34 | - | CDS 3' incomplete, |
| GSS-218 | ENST00000645102.1 | 512 | 112aa | Protein coding | - | A0A2R8Y790 | - | CDS 3' incomplete, |
| GSS-215 | ENST00000644608.1 | 450 | 30aa | Protein coding | - | A0A2R8Y2F2 | - | CDS 3' incomplete, |
| GSS-222 | ENST00000646405.1 | 1667 | 124aa | Nonsense mediated decay | - | A0A2R8Y446 | - | - |
| GSS-204 | ENST00000642538.1 | 1540 | 132aa | Nonsense mediated decay | - | A0A0S2Z4J7 | - | - |
| GSS-208 | ENST00000643443.1 | 1437 | 81aa | Nonsense mediated decay | - | A0A2R8Y6Y6 | - | - |
| GSS-220 | ENST00000645408.1 | 830 | 155aa | Nonsense mediated decay | - | A0A2R8Y7I7 | - | CDS 5' incomplete, |
| GSS-202 | ENST00000642493.1 | 767 | 48aa | Nonsense mediated decay | - | A0A2R8Y2X9 | - | - |
| GSS-227 | ENST00000646766.1 | 732 | 97aa | Nonsense mediated decay | - | A0A2R8Y4V9 | - | - |
| GSS-224 | ENST00000646502.1 | 1870 | No protein | Processed transcript | - | - | - | - |
| GSS-210 | ENST00000643628.1 | 920 | No protein | Processed transcript | - | - | - | - |
| GSS-223 | ENST00000646497.1 | 900 | No protein | Processed transcript | - | - | - | - |
| GSS-221 | ENST00000645723.1 | 3019 | No protein | Retained intron | - | - | - | - |
| GSS-212 | ENST00000643908.1 | 1998 | No protein | Retained intron | - | - | - | - |
| GSS-225 | ENST00000646512.1 | 1907 | No protein | Retained intron | - | - | - | - |
| GSS-214 | ENST00000644538.1 | 1780 | No protein | Retained intron | - | - | - | - |
| GSS-216 | ENST00000644694.1 | 747 | No protein | Retained intron | - | - | - | - |
| GSS-213 | ENST00000644197.1 | 728 | No protein | Retained intron | - | - | - | - |
| GSS-211 | ENST00000643690.1 | 573 | No protein | Retained intron | - | - | - | - |
| GSS-206 | ENST00000643203.1 | 461 | No protein | Retained intron | - | - | - | - |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | GSS |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene GSS had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
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Work flow
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