CRISPR-U™ Gene Knockout Cell Line
Strategy
CXCL1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CXCL1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CXCL1 |
| Gene id | 2919 |
| Organism | Homo sapiens |
| Official full symbol | C-X-C motif chemokine ligand 1 |
| Gene type | protein-coding |
| Also known as | FSP, GRO1, GROa, MGSA, MGSA-a, NAP-3, SCYB1 |
| Summary | This antimicrobial gene encodes a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signals through the G-protein coupled receptor, CXC receptor 2. This protein plays a role in inflammation and as a chemoattractant for neutrophils. Aberrant expression of this protein is associated with the growth and progression of certain tumors. A naturally occurring processed form of this protein has increased chemotactic activity. Alternate splicing results in coding and non-coding variants of this gene. A pseudogene of this gene is found on chromosome 4. |
| Genomic regions | Chromosome 4 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CXCL1-201 | ENST00000395761.4 | 1174 | 107aa | Protein coding | CCDS47074 | P09341 | NM_001511.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CXCL1-202 | ENST00000509101.1 | 574 | No protein | Processed transcript | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CXCL1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CXCL1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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