CRISPR-U™ Gene Knockout Cell Line
Strategy
DROSHA Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human DROSHA Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | DROSHA |
| Gene id | 29102 |
| Organism | Homo sapiens |
| Official full symbol | drosha ribonuclease III |
| Gene type | protein-coding |
| Also known as | ETOHI2, HSA242976, RANSE3L, RN3, RNASE3L, RNASEN |
| Summary | This gene encodes a ribonuclease (RNase) III double-stranded RNA-specific ribonuclease and subunit of the microprocessor protein complex, which catalyzes the initial processing step of microRNA (miRNA) synthesis. The encoded protein cleaves the stem loop structure from the primary microRNA (pri-miRNA) in the nucleus, yielding the precursor miRNA (pre-miRNA), which is then exported to the cytoplasm for further processing. In a human cell line lacking a functional copy of this gene, canonical miRNA synthesis is reduced. Somatic mutations in this gene have been observed in human patients with kidney cancer. |
| Genomic regions | Chromosome 5 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| DROSHA-201 | ENST00000344624.8 | 5416 | 1374aa | Protein coding | CCDS47195 | Q9NRR4-1 | NM_001382508.1 | TSL:5, GENCODE basic, APPRIS ALT1, MANE Select v0.92, |
| DROSHA-211 | ENST00000511367.6 | 5305 | 1374aa | Protein coding | CCDS47195 | Q9NRR4-1 | - | TSL:1, GENCODE basic, APPRIS ALT1, |
| DROSHA-219 | ENST00000513349.5 | 4681 | 1337aa | Protein coding | CCDS47194 | Q9NRR4-4 | - | TSL:1, GENCODE basic, APPRIS P4, |
| DROSHA-214 | ENST00000512076.1 | 1124 | 375aa | Protein coding | - | H7C5U6 | - | CDS 5' and 3' incomplete, TSL:1, |
| DROSHA-206 | ENST00000507438.5 | 860 | 201aa | Protein coding | - | E5RHD1 | - | CDS 3' incomplete, TSL:4, |
| DROSHA-217 | ENST00000512302.1 | 585 | 195aa | Protein coding | - | H0YB67 | - | CDS 5' and 3' incomplete, TSL:4, |
| DROSHA-203 | ENST00000504361.1 | 583 | No protein | Processed transcript | - | - | - | TSL:3, |
| DROSHA-210 | ENST00000510375.5 | 579 | No protein | Processed transcript | - | - | - | TSL:4, |
| DROSHA-215 | ENST00000512124.6 | 555 | No protein | Processed transcript | - | - | - | TSL:5, |
| DROSHA-204 | ENST00000505601.5 | 552 | No protein | Processed transcript | - | - | - | TSL:4, |
| DROSHA-218 | ENST00000512885.1 | 437 | No protein | Processed transcript | - | - | - | TSL:4, |
| DROSHA-209 | ENST00000510178.1 | 379 | No protein | Processed transcript | - | - | - | TSL:5, |
| DROSHA-212 | ENST00000511778.5 | 1261 | No protein | Retained intron | - | - | - | TSL:3, |
| DROSHA-207 | ENST00000509067.1 | 726 | No protein | Retained intron | - | - | - | TSL:2, |
| DROSHA-213 | ENST00000511803.1 | 717 | No protein | Retained intron | - | - | - | TSL:2, |
| DROSHA-202 | ENST00000504133.5 | 617 | No protein | Retained intron | - | - | - | TSL:3, |
| DROSHA-205 | ENST00000507174.1 | 572 | No protein | Retained intron | - | - | - | TSL:4, |
| DROSHA-220 | ENST00000514927.6 | 567 | No protein | Retained intron | - | - | - | TSL:2, |
| DROSHA-208 | ENST00000509608.1 | 409 | No protein | Retained intron | - | - | - | TSL:3, |
| DROSHA-216 | ENST00000512166.2 | 257 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | DROSHA |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene DROSHA had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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