CRISPR-U™ Gene Knockout Cell Line Strategy

CNIH4 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CNIH4 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CNIH4
Gene id 29097
Organism Homo sapiens
Official full symbol cornichon family AMPA receptor auxiliary protein 4
Gene type protein-coding
Also known as CNIH-4, CNIH2, HSPC163
Genomic regions Chromosome 1
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CNIH4-205 ENST00000465271.6 4096 139aa Protein coding CCDS1543 Q9P003-1 NM_014184.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CNIH4-202 ENST00000366857.9 3963 92aa Protein coding CCDS60430 Q9P003-2 - TSL:1, GENCODE basic,
CNIH4-201 ENST00000366856.3 808 137aa Protein coding CCDS60429 A6NLH6 - TSL:2, GENCODE basic,
CNIH4-203 ENST00000366858.7 702 90aa Protein coding - A6NJ96 - TSL:3, GENCODE basic,
CNIH4-206 ENST00000468318.5 1525 No protein Processed transcript - - - TSL:2,
CNIH4-204 ENST00000366860.9 912 No protein Processed transcript - - - TSL:3,
CNIH4-208 ENST00000477413.1 820 No protein Processed transcript - - - TSL:5,
CNIH4-207 ENST00000469200.1 518 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CNIH4
This KO Strategy loading
Red Cotton™ Notes Gene CNIH4 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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$49

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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