CRISPR-U™ Gene Knockout Cell Line Strategy

CHMP4A Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CHMP4A Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CHMP4A
Gene id 29082
Organism Homo sapiens
Official full symbol charged multivesicular body protein 4A
Gene type protein-coding
Also known as C14orf123, CHMP4, CHMP4B, HSPC134, SHAX2, SNF7, SNF7-1, VPS32-1, VPS32A
Summary CHMP4A belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 ).
Genomic regions Chromosome 14
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CHMP4A-201 ENST00000347519.12 980 222aa Protein coding - Q9BY43-1 NM_014169.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CHMP4A-204 ENST00000530996.5 967 117aa Protein coding - H0YIN6 - TSL:3, GENCODE basic,
CHMP4A-206 ENST00000533011.5 733 213aa Protein coding - E9PQ80 - CDS 3' incomplete, TSL:5,
CHMP4A-207 ENST00000533523.6 1266 123aa Nonsense mediated decay - E9PQI5 - TSL:1,
CHMP4A-208 ENST00000534106.2 370 61aa Nonsense mediated decay - H0YI89 - CDS 5' incomplete, TSL:5,
CHMP4A-209 ENST00000542700.6 847 No protein Processed transcript - - - TSL:3,
CHMP4A-205 ENST00000531158.1 563 No protein Processed transcript - - - TSL:4,
CHMP4A-210 ENST00000552620.1 716 No protein Retained intron - - - TSL:2,
CHMP4A-203 ENST00000527154.5 571 No protein Retained intron - - - TSL:2,
CHMP4A-202 ENST00000524955.1 268 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CHMP4A
This KO Strategy loading
Red Cotton™ Notes Gene CHMP4A had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×