CRISPR-U™ Gene Knockout Cell Line
Strategy
CHMP4A Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CHMP4A Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CHMP4A |
| Gene id | 29082 |
| Organism | Homo sapiens |
| Official full symbol | charged multivesicular body protein 4A |
| Gene type | protein-coding |
| Also known as | C14orf123, CHMP4, CHMP4B, HSPC134, SHAX2, SNF7, SNF7-1, VPS32-1, VPS32A |
| Summary | CHMP4A belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 ). |
| Genomic regions | Chromosome 14 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CHMP4A-201 | ENST00000347519.12 | 980 | 222aa | Protein coding | - | Q9BY43-1 | NM_014169.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CHMP4A-204 | ENST00000530996.5 | 967 | 117aa | Protein coding | - | H0YIN6 | - | TSL:3, GENCODE basic, |
| CHMP4A-206 | ENST00000533011.5 | 733 | 213aa | Protein coding | - | E9PQ80 | - | CDS 3' incomplete, TSL:5, |
| CHMP4A-207 | ENST00000533523.6 | 1266 | 123aa | Nonsense mediated decay | - | E9PQI5 | - | TSL:1, |
| CHMP4A-208 | ENST00000534106.2 | 370 | 61aa | Nonsense mediated decay | - | H0YI89 | - | CDS 5' incomplete, TSL:5, |
| CHMP4A-209 | ENST00000542700.6 | 847 | No protein | Processed transcript | - | - | - | TSL:3, |
| CHMP4A-205 | ENST00000531158.1 | 563 | No protein | Processed transcript | - | - | - | TSL:4, |
| CHMP4A-210 | ENST00000552620.1 | 716 | No protein | Retained intron | - | - | - | TSL:2, |
| CHMP4A-203 | ENST00000527154.5 | 571 | No protein | Retained intron | - | - | - | TSL:2, |
| CHMP4A-202 | ENST00000524955.1 | 268 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CHMP4A |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CHMP4A had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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