CRISPR-U™ Gene Knockout Cell Line Strategy

METTL5 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human METTL5 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol METTL5
Gene id 29081
Organism Homo sapiens
Official full symbol methyltransferase like 5
Gene type protein-coding
Also known as HSPC133, MRT72
Genomic regions Chromosome 2
Strategy Summary
This gene has 9 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
METTL5-201 ENST00000260953.10 1001 209aa Protein coding CCDS33320 Q9NRN9 NM_014168.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
METTL5-206 ENST00000409965.5 880 209aa Protein coding CCDS33320 Q9NRN9 - TSL:1, GENCODE basic, APPRIS P1,
METTL5-203 ENST00000392640.6 800 209aa Protein coding CCDS33320 Q9NRN9 - TSL:1, GENCODE basic, APPRIS P1,
METTL5-207 ENST00000410097.5 1370 244aa Protein coding - B8ZZC8 - TSL:2, GENCODE basic,
METTL5-205 ENST00000409837.5 936 198aa Protein coding - B8ZZE3 - TSL:1, GENCODE basic,
METTL5-202 ENST00000308099.7 629 164aa Protein coding - E7EMN2 - TSL:3, GENCODE basic,
METTL5-204 ENST00000409340.5 472 110aa Protein coding - B8ZZY8 - TSL:5, GENCODE basic,
METTL5-208 ENST00000442181.5 443 126aa Protein coding - H7C0M7 - CDS 5' incomplete, TSL:2,
METTL5-212 ENST00000538491.1 235 48aa Protein coding - F5H4E8 - CDS 3' incomplete, TSL:5,
METTL5-211 ENST00000537825.5 464 87aa Nonsense mediated decay - H0YFV5 - CDS 5' incomplete, TSL:3,
METTL5-210 ENST00000484351.1 820 No protein Retained intron - - - TSL:2,
METTL5-209 ENST00000471560.1 550 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene METTL5
This KO Strategy loading
Red Cotton™ Notes Gene METTL5 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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