CRISPR-U™ Gene Knockout Cell Line
Strategy
NR3C1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NR3C1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NR3C1 |
| Gene id | 2908 |
| Organism | Homo sapiens |
| Official full symbol | nuclear receptor subfamily 3 group C member 1 |
| Gene type | protein-coding |
| Also known as | GCCR, GCR, GCRST, GR, GRL |
| Summary | This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175). |
| Genomic regions | Chromosome 5 |
Strategy Summary
This gene has 14 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NR3C1-202 | ENST00000343796.6 | 7286 | 777aa | Protein coding | CCDS4278 | P04150-1 | - | TSL:5, GENCODE basic, APPRIS P4, |
| NR3C1-203 | ENST00000394464.7 | 6778 | 777aa | Protein coding | CCDS4278 | P04150-1 | NM_000176.3 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| NR3C1-205 | ENST00000415690.6 | 3789 | 742aa | Protein coding | CCDS47298 | P04150-2 | - | TSL:1, GENCODE basic, |
| NR3C1-201 | ENST00000231509.7 | 3556 | 778aa | Protein coding | CCDS34258 | P04150-3 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| NR3C1-212 | ENST00000504572.5 | 3389 | 778aa | Protein coding | CCDS34258 | P04150-3 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| NR3C1-204 | ENST00000394466.6 | 3245 | 778aa | Protein coding | CCDS34258 | P04150-3 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| NR3C1-209 | ENST00000503201.1 | 2594 | 777aa | Protein coding | CCDS4278 | P04150-1 | - | TSL:1, GENCODE basic, APPRIS P4, |
| NR3C1-206 | ENST00000424646.6 | 4591 | 751aa | Protein coding | - | P04150-10 | - | TSL:1, GENCODE basic, |
| NR3C1-217 | ENST00000652686.1 | 2961 | 746aa | Protein coding | - | A0A494C0P1 | - | GENCODE basic, |
| NR3C1-216 | ENST00000514699.1 | 623 | 145aa | Protein coding | - | Q3MSN4 | - | CDS 3' incomplete, TSL:1, |
| NR3C1-207 | ENST00000502500.1 | 585 | 144aa | Protein coding | - | D6RDA9 | - | CDS 3' incomplete, TSL:4, |
| NR3C1-215 | ENST00000510170.5 | 585 | 145aa | Protein coding | - | Q3MSN4 | - | CDS 3' incomplete, TSL:1, |
| NR3C1-214 | ENST00000508760.5 | 547 | 145aa | Protein coding | - | Q3MSN4 | - | CDS 3' incomplete, TSL:1, |
| NR3C1-208 | ENST00000502892.5 | 498 | 145aa | Protein coding | - | Q3MSN4 | - | CDS 3' incomplete, TSL:1, |
| NR3C1-211 | ENST00000504336.1 | 611 | No protein | Processed transcript | - | - | - | TSL:2, |
| NR3C1-210 | ENST00000503701.1 | 602 | No protein | Processed transcript | - | - | - | TSL:3, |
| NR3C1-213 | ENST00000505058.5 | 491 | No protein | Processed transcript | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NR3C1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NR3C1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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