CRISPR-U™ Gene Knockout Cell Line Strategy

NR3C1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NR3C1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NR3C1
Gene id 2908
Organism Homo sapiens
Official full symbol nuclear receptor subfamily 3 group C member 1
Gene type protein-coding
Also known as GCCR, GCR, GCRST, GR, GRL
Summary This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175).
Genomic regions Chromosome 5
Strategy Summary
This gene has 14 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NR3C1-202 ENST00000343796.6 7286 777aa Protein coding CCDS4278 P04150-1 - TSL:5, GENCODE basic, APPRIS P4,
NR3C1-203 ENST00000394464.7 6778 777aa Protein coding CCDS4278 P04150-1 NM_000176.3 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
NR3C1-205 ENST00000415690.6 3789 742aa Protein coding CCDS47298 P04150-2 - TSL:1, GENCODE basic,
NR3C1-201 ENST00000231509.7 3556 778aa Protein coding CCDS34258 P04150-3 - TSL:1, GENCODE basic, APPRIS ALT2,
NR3C1-212 ENST00000504572.5 3389 778aa Protein coding CCDS34258 P04150-3 - TSL:1, GENCODE basic, APPRIS ALT2,
NR3C1-204 ENST00000394466.6 3245 778aa Protein coding CCDS34258 P04150-3 - TSL:5, GENCODE basic, APPRIS ALT2,
NR3C1-209 ENST00000503201.1 2594 777aa Protein coding CCDS4278 P04150-1 - TSL:1, GENCODE basic, APPRIS P4,
NR3C1-206 ENST00000424646.6 4591 751aa Protein coding - P04150-10 - TSL:1, GENCODE basic,
NR3C1-217 ENST00000652686.1 2961 746aa Protein coding - A0A494C0P1 - GENCODE basic,
NR3C1-216 ENST00000514699.1 623 145aa Protein coding - Q3MSN4 - CDS 3' incomplete, TSL:1,
NR3C1-207 ENST00000502500.1 585 144aa Protein coding - D6RDA9 - CDS 3' incomplete, TSL:4,
NR3C1-215 ENST00000510170.5 585 145aa Protein coding - Q3MSN4 - CDS 3' incomplete, TSL:1,
NR3C1-214 ENST00000508760.5 547 145aa Protein coding - Q3MSN4 - CDS 3' incomplete, TSL:1,
NR3C1-208 ENST00000502892.5 498 145aa Protein coding - Q3MSN4 - CDS 3' incomplete, TSL:1,
NR3C1-211 ENST00000504336.1 611 No protein Processed transcript - - - TSL:2,
NR3C1-210 ENST00000503701.1 602 No protein Processed transcript - - - TSL:3,
NR3C1-213 ENST00000505058.5 491 No protein Processed transcript - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NR3C1
This KO Strategy loading
Red Cotton™ Notes Gene NR3C1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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