CRISPR-U™ Gene Knockout Cell Line Strategy

GIT1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human GIT1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol GIT1
Gene id 28964
Organism Homo sapiens
Official full symbol GIT ArfGAP 1
Gene type protein-coding
Genomic regions Chromosome 17
Strategy Summary
This gene has 9 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
GIT1-201 ENST00000225394.8 3783 761aa Protein coding CCDS11250 Q9Y2X7-1 NM_014030.4 TSL:1, GENCODE basic, APPRIS ALT1, MANE Select v0.92,
GIT1-203 ENST00000394869.7 3711 770aa Protein coding CCDS42290 Q9Y2X7-3 - TSL:1, GENCODE basic, APPRIS P3,
GIT1-211 ENST00000581348.5 2827 747aa Protein coding - A0A0C4DGN6 - TSL:2, GENCODE basic,
GIT1-210 ENST00000579937.5 2798 694aa Protein coding - J3QRU8 - TSL:2, GENCODE basic,
GIT1-214 ENST00000585148.1 686 228aa Protein coding - J3QLH1 - CDS 5' and 3' incomplete, TSL:3,
GIT1-202 ENST00000335356.7 660 147aa Protein coding - E7EVC2 - CDS 3' incomplete, TSL:3,
GIT1-207 ENST00000578266.1 555 177aa Protein coding - J3QL89 - CDS 5' incomplete, TSL:5,
GIT1-206 ENST00000577466.2 547 52aa Protein coding - K7EIX6 - CDS 3' incomplete, TSL:2,
GIT1-213 ENST00000583413.4 546 173aa Protein coding - K7EN79 - CDS 3' incomplete, TSL:4,
GIT1-208 ENST00000578670.5 918 149aa Nonsense mediated decay - J3QQI0 - CDS 5' incomplete, TSL:3,
GIT1-204 ENST00000473217.5 3462 No protein Retained intron - - - TSL:1,
GIT1-209 ENST00000579536.1 852 No protein Retained intron - - - TSL:2,
GIT1-205 ENST00000491377.1 790 No protein Retained intron - - - TSL:5,
GIT1-212 ENST00000581925.1 610 No protein Retained intron - - - TSL:4,
GIT1-215 ENST00000586574.5 576 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene GIT1
This KO Strategy loading
Red Cotton™ Notes Gene GIT1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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