CRISPR-U™ Gene Knockout Cell Line
Strategy
GIT1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human GIT1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | GIT1 |
| Gene id | 28964 |
| Organism | Homo sapiens |
| Official full symbol | GIT ArfGAP 1 |
| Gene type | protein-coding |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 9 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| GIT1-201 | ENST00000225394.8 | 3783 | 761aa | Protein coding | CCDS11250 | Q9Y2X7-1 | NM_014030.4 | TSL:1, GENCODE basic, APPRIS ALT1, MANE Select v0.92, |
| GIT1-203 | ENST00000394869.7 | 3711 | 770aa | Protein coding | CCDS42290 | Q9Y2X7-3 | - | TSL:1, GENCODE basic, APPRIS P3, |
| GIT1-211 | ENST00000581348.5 | 2827 | 747aa | Protein coding | - | A0A0C4DGN6 | - | TSL:2, GENCODE basic, |
| GIT1-210 | ENST00000579937.5 | 2798 | 694aa | Protein coding | - | J3QRU8 | - | TSL:2, GENCODE basic, |
| GIT1-214 | ENST00000585148.1 | 686 | 228aa | Protein coding | - | J3QLH1 | - | CDS 5' and 3' incomplete, TSL:3, |
| GIT1-202 | ENST00000335356.7 | 660 | 147aa | Protein coding | - | E7EVC2 | - | CDS 3' incomplete, TSL:3, |
| GIT1-207 | ENST00000578266.1 | 555 | 177aa | Protein coding | - | J3QL89 | - | CDS 5' incomplete, TSL:5, |
| GIT1-206 | ENST00000577466.2 | 547 | 52aa | Protein coding | - | K7EIX6 | - | CDS 3' incomplete, TSL:2, |
| GIT1-213 | ENST00000583413.4 | 546 | 173aa | Protein coding | - | K7EN79 | - | CDS 3' incomplete, TSL:4, |
| GIT1-208 | ENST00000578670.5 | 918 | 149aa | Nonsense mediated decay | - | J3QQI0 | - | CDS 5' incomplete, TSL:3, |
| GIT1-204 | ENST00000473217.5 | 3462 | No protein | Retained intron | - | - | - | TSL:1, |
| GIT1-209 | ENST00000579536.1 | 852 | No protein | Retained intron | - | - | - | TSL:2, |
| GIT1-205 | ENST00000491377.1 | 790 | No protein | Retained intron | - | - | - | TSL:5, |
| GIT1-212 | ENST00000581925.1 | 610 | No protein | Retained intron | - | - | - | TSL:4, |
| GIT1-215 | ENST00000586574.5 | 576 | No protein | Retained intron | - | - | - | TSL:4, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | GIT1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene GIT1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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