CRISPR-U™ Gene Knockout Cell Line Strategy

MRPS28 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MRPS28 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MRPS28
Gene id 28957
Organism Homo sapiens
Official full symbol mitochondrial ribosomal protein S28
Gene type protein-coding
Also known as COXPD47, HSPC007, MRP-S28, MRP-S35, MRPS35
Summary Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that has been called mitochondrial ribosomal protein S35 in the literature.
Genomic regions Chromosome 8
Strategy Summary
This gene has 8 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MRPS28-201 ENST00000276585.9 838 187aa Protein coding CCDS6226 A0A0S2Z563 Q9Y2Q9 NM_014018.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
MRPS28-203 ENST00000518371.2 783 83aa Protein coding - U3KQQ8 - CDS 5' incomplete, TSL:3,
MRPS28-207 ENST00000521434.5 585 125aa Protein coding - E5RFH3 - TSL:5, GENCODE basic,
MRPS28-205 ENST00000519386.5 555 143aa Protein coding - H0YAT2 - CDS 5' incomplete, TSL:2,
MRPS28-202 ENST00000518271.1 487 162aa Protein coding - H7C5V3 - CDS 5' and 3' incomplete, TSL:5,
MRPS28-208 ENST00000521605.1 437 86aa Protein coding - E5RFT8 - TSL:2, GENCODE basic,
MRPS28-206 ENST00000520946.1 411 108aa Protein coding - E5RK86 - CDS 3' incomplete, TSL:5,
MRPS28-204 ENST00000519120.1 403 103aa Protein coding - E5RGC7 - CDS 3' incomplete, TSL:2,
MRPS28-209 ENST00000522987.1 487 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MRPS28
This KO Strategy loading
Red Cotton™ Notes Gene MRPS28 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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