CRISPR-U™ Gene Knockout Cell Line Strategy

LAMTOR2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human LAMTOR2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol LAMTOR2
Gene id 28956
Organism Homo sapiens
Official full symbol late endosomal/lysosomal adaptor, MAPK and MTOR activator 2
Gene type protein-coding
Also known as ENDAP, HSPC003, MAPBPIP, MAPKSP1AP, ROBLD3, Ragulator2, p14
Summary The product of this gene is highly conserved with a mouse protein associated with the cytoplasmic face of late endosomes and lysosomes. The mouse protein interacts with MAPK scaffold protein 1, a component of the mitogen-activated protein kinase pathway. In humans, a mutation in this gene has been associated with a primary immunodeficiency syndrome, and suggests a role for this protein in endosomal biogenesis. Multiple transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 1
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
LAMTOR2-203 ENST00000368305.9 621 125aa Protein coding CCDS1128 Q9Y2Q5-1 NM_014017.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
LAMTOR2-202 ENST00000368304.9 521 95aa Protein coding CCDS44243 Q9Y2Q5-3 - TSL:2, GENCODE basic,
LAMTOR2-201 ENST00000368302.3 587 150aa Protein coding - Q9Y2Q5-2 - TSL:3, GENCODE basic,
LAMTOR2-206 ENST00000489664.1 868 No protein Processed transcript - - - TSL:2,
LAMTOR2-205 ENST00000487106.5 623 No protein Processed transcript - - - TSL:2,
LAMTOR2-204 ENST00000463371.1 462 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene LAMTOR2
This KO Strategy loading
Red Cotton™ Notes Gene LAMTOR2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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