CRISPR-U™ Gene Knockout Cell Line
Strategy
LAMTOR2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human LAMTOR2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | LAMTOR2 |
| Gene id | 28956 |
| Organism | Homo sapiens |
| Official full symbol | late endosomal/lysosomal adaptor, MAPK and MTOR activator 2 |
| Gene type | protein-coding |
| Also known as | ENDAP, HSPC003, MAPBPIP, MAPKSP1AP, ROBLD3, Ragulator2, p14 |
| Summary | The product of this gene is highly conserved with a mouse protein associated with the cytoplasmic face of late endosomes and lysosomes. The mouse protein interacts with MAPK scaffold protein 1, a component of the mitogen-activated protein kinase pathway. In humans, a mutation in this gene has been associated with a primary immunodeficiency syndrome, and suggests a role for this protein in endosomal biogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| LAMTOR2-203 | ENST00000368305.9 | 621 | 125aa | Protein coding | CCDS1128 | Q9Y2Q5-1 | NM_014017.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| LAMTOR2-202 | ENST00000368304.9 | 521 | 95aa | Protein coding | CCDS44243 | Q9Y2Q5-3 | - | TSL:2, GENCODE basic, |
| LAMTOR2-201 | ENST00000368302.3 | 587 | 150aa | Protein coding | - | Q9Y2Q5-2 | - | TSL:3, GENCODE basic, |
| LAMTOR2-206 | ENST00000489664.1 | 868 | No protein | Processed transcript | - | - | - | TSL:2, |
| LAMTOR2-205 | ENST00000487106.5 | 623 | No protein | Processed transcript | - | - | - | TSL:2, |
| LAMTOR2-204 | ENST00000463371.1 | 462 | No protein | Processed transcript | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | LAMTOR2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene LAMTOR2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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