CRISPR-U™ Gene Knockout Cell Line Strategy

MKNK2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MKNK2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MKNK2
Gene id 2872
Organism Homo sapiens
Official full symbol MAPK interacting serine/threonine kinase 2
Gene type protein-coding
Also known as GPRK7, MNK2
Summary This gene encodes a member of the calcium/calmodulin-dependent protein kinases (CAMK) Ser/Thr protein kinase family, which belongs to the protein kinase superfamily. This protein contains conserved DLG (asp-leu-gly) and ENIL (glu-asn-ile-leu) motifs, and an N-terminal polybasic region which binds importin A and the translation factor scaffold protein eukaryotic initiation factor 4G (eIF4G). This protein is one of the downstream kinases activated by mitogen-activated protein (MAP) kinases. It phosphorylates the eukaryotic initiation factor 4E (eIF4E), thus playing important roles in the initiation of mRNA translation, oncogenic transformation and malignant cell proliferation. In addition to eIF4E, this protein also interacts with von Hippel-Lindau tumor suppressor (VHL), ring-box 1 (Rbx1) and Cullin2 (Cul2), which are all components of the CBC(VHL) ubiquitin ligase E3 complex. Multiple alternatively spliced transcript variants have been found, but the full-length nature and biological activity of only two variants are determined. These two variants encode distinct isoforms which differ in activity and regulation, and in subcellular localization.
Genomic regions Chromosome 19
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MKNK2-201 ENST00000250896.9 3785 465aa Protein coding CCDS12080 Q9HBH9 NM_199054.3 TSL:5, GENCODE basic, APPRIS P1, MANE Select v0.92,
MKNK2-202 ENST00000309340.11 1744 414aa Protein coding CCDS12079 - - TSL:1, GENCODE basic,
MKNK2-207 ENST00000588014.5 2413 176aa Protein coding - K7EJ98 - CDS 5' incomplete, TSL:2,
MKNK2-211 ENST00000591142.5 875 158aa Protein coding - Q9NV89 - TSL:2, GENCODE basic,
MKNK2-212 ENST00000591588.1 631 129aa Protein coding - K7EIN7 - TSL:2, GENCODE basic,
MKNK2-210 ENST00000589534.2 454 73aa Protein coding - K7ELG3 - CDS 3' incomplete, TSL:2,
MKNK2-209 ENST00000589509.5 352 76aa Protein coding - K7ELN0 - CDS 3' incomplete, TSL:5,
MKNK2-205 ENST00000586828.5 1503 82aa Nonsense mediated decay - K7EK27 - TSL:2,
MKNK2-208 ENST00000589441.5 803 43aa Nonsense mediated decay - K7EMP5 - CDS 5' incomplete, TSL:5,
MKNK2-206 ENST00000587416.5 4093 No protein Retained intron - - - TSL:2,
MKNK2-204 ENST00000586620.2 1135 No protein Retained intron - - - TSL:2,
MKNK2-203 ENST00000585667.1 870 No protein Retained intron - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MKNK2
This KO Strategy loading
Red Cotton™ Notes Gene MKNK2 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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