CRISPR-U™ Gene Knockout Cell Line
Strategy
MKNK2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MKNK2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MKNK2 |
| Gene id | 2872 |
| Organism | Homo sapiens |
| Official full symbol | MAPK interacting serine/threonine kinase 2 |
| Gene type | protein-coding |
| Also known as | GPRK7, MNK2 |
| Summary | This gene encodes a member of the calcium/calmodulin-dependent protein kinases (CAMK) Ser/Thr protein kinase family, which belongs to the protein kinase superfamily. This protein contains conserved DLG (asp-leu-gly) and ENIL (glu-asn-ile-leu) motifs, and an N-terminal polybasic region which binds importin A and the translation factor scaffold protein eukaryotic initiation factor 4G (eIF4G). This protein is one of the downstream kinases activated by mitogen-activated protein (MAP) kinases. It phosphorylates the eukaryotic initiation factor 4E (eIF4E), thus playing important roles in the initiation of mRNA translation, oncogenic transformation and malignant cell proliferation. In addition to eIF4E, this protein also interacts with von Hippel-Lindau tumor suppressor (VHL), ring-box 1 (Rbx1) and Cullin2 (Cul2), which are all components of the CBC(VHL) ubiquitin ligase E3 complex. Multiple alternatively spliced transcript variants have been found, but the full-length nature and biological activity of only two variants are determined. These two variants encode distinct isoforms which differ in activity and regulation, and in subcellular localization. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MKNK2-201 | ENST00000250896.9 | 3785 | 465aa | Protein coding | CCDS12080 | Q9HBH9 | NM_199054.3 | TSL:5, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| MKNK2-202 | ENST00000309340.11 | 1744 | 414aa | Protein coding | CCDS12079 | - | - | TSL:1, GENCODE basic, |
| MKNK2-207 | ENST00000588014.5 | 2413 | 176aa | Protein coding | - | K7EJ98 | - | CDS 5' incomplete, TSL:2, |
| MKNK2-211 | ENST00000591142.5 | 875 | 158aa | Protein coding | - | Q9NV89 | - | TSL:2, GENCODE basic, |
| MKNK2-212 | ENST00000591588.1 | 631 | 129aa | Protein coding | - | K7EIN7 | - | TSL:2, GENCODE basic, |
| MKNK2-210 | ENST00000589534.2 | 454 | 73aa | Protein coding | - | K7ELG3 | - | CDS 3' incomplete, TSL:2, |
| MKNK2-209 | ENST00000589509.5 | 352 | 76aa | Protein coding | - | K7ELN0 | - | CDS 3' incomplete, TSL:5, |
| MKNK2-205 | ENST00000586828.5 | 1503 | 82aa | Nonsense mediated decay | - | K7EK27 | - | TSL:2, |
| MKNK2-208 | ENST00000589441.5 | 803 | 43aa | Nonsense mediated decay | - | K7EMP5 | - | CDS 5' incomplete, TSL:5, |
| MKNK2-206 | ENST00000587416.5 | 4093 | No protein | Retained intron | - | - | - | TSL:2, |
| MKNK2-204 | ENST00000586620.2 | 1135 | No protein | Retained intron | - | - | - | TSL:2, |
| MKNK2-203 | ENST00000585667.1 | 870 | No protein | Retained intron | - | - | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MKNK2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MKNK2 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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