CRISPR-U™ Gene Knockout Cell Line Strategy

GRK4 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human GRK4 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol GRK4
Gene id 2868
Organism Homo sapiens
Official full symbol G protein-coupled receptor kinase 4
Gene type protein-coding
Also known as GPRK2L, GPRK4, GRK4a, IT11
Summary This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating its deactivation. This gene has been linked to both genetic and acquired hypertension. Several transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 4
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
GRK4-203 ENST00000398052.9 2372 578aa Protein coding CCDS33946 P32298-1 NM_182982.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
GRK4-202 ENST00000398051.8 2068 500aa Protein coding CCDS68656 P32298-3 - TSL:1, GENCODE basic,
GRK4-201 ENST00000345167.10 2013 546aa Protein coding CCDS47002 P32298-2 - TSL:1, GENCODE basic,
GRK4-206 ENST00000504933.1 1971 532aa Protein coding CCDS33947 P32298-4 - TSL:1, GENCODE basic,
GRK4-204 ENST00000503518.2 2136 84aa Protein coding - D6REW5 - TSL:1, GENCODE basic,
GRK4-209 ENST00000509545.5 831 No protein Processed transcript - - - TSL:3,
GRK4-210 ENST00000511827.5 614 No protein Processed transcript - - - TSL:5,
GRK4-207 ENST00000505271.5 515 No protein Processed transcript - - - TSL:4,
GRK4-205 ENST00000504308.1 428 No protein Processed transcript - - - TSL:3,
GRK4-208 ENST00000507230.1 394 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene GRK4
This KO Strategy loading
Red Cotton™ Notes Gene GRK4 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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