CRISPR-U™ Gene Knockout Cell Line
Strategy
GRK4 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human GRK4 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | GRK4 |
| Gene id | 2868 |
| Organism | Homo sapiens |
| Official full symbol | G protein-coupled receptor kinase 4 |
| Gene type | protein-coding |
| Also known as | GPRK2L, GPRK4, GRK4a, IT11 |
| Summary | This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating its deactivation. This gene has been linked to both genetic and acquired hypertension. Several transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 4 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| GRK4-203 | ENST00000398052.9 | 2372 | 578aa | Protein coding | CCDS33946 | P32298-1 | NM_182982.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| GRK4-202 | ENST00000398051.8 | 2068 | 500aa | Protein coding | CCDS68656 | P32298-3 | - | TSL:1, GENCODE basic, |
| GRK4-201 | ENST00000345167.10 | 2013 | 546aa | Protein coding | CCDS47002 | P32298-2 | - | TSL:1, GENCODE basic, |
| GRK4-206 | ENST00000504933.1 | 1971 | 532aa | Protein coding | CCDS33947 | P32298-4 | - | TSL:1, GENCODE basic, |
| GRK4-204 | ENST00000503518.2 | 2136 | 84aa | Protein coding | - | D6REW5 | - | TSL:1, GENCODE basic, |
| GRK4-209 | ENST00000509545.5 | 831 | No protein | Processed transcript | - | - | - | TSL:3, |
| GRK4-210 | ENST00000511827.5 | 614 | No protein | Processed transcript | - | - | - | TSL:5, |
| GRK4-207 | ENST00000505271.5 | 515 | No protein | Processed transcript | - | - | - | TSL:4, |
| GRK4-205 | ENST00000504308.1 | 428 | No protein | Processed transcript | - | - | - | TSL:3, |
| GRK4-208 | ENST00000507230.1 | 394 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | GRK4 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene GRK4 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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