CRISPR-U™ Gene Knockout Cell Line Strategy

NOMO2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human NOMO2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NOMO2
Gene id 283820
Organism Homo sapiens
Official full symbol NODAL modulator 2
Gene type protein-coding
Also known as Nomo, PM5
Summary This gene encodes a protein originally thought to be related to the collagenase gene family. This gene is one of three highly similar genes in a region of duplication located on the p arm of chromosome 16. These three genes encode closely related proteins that may have the same function. The protein encoded by one of these genes has been identified as part of a protein complex that participates in the Nodal signaling pathway during vertebrate development. Mutations in ABCC6, which is located nearby, rather than mutations in this gene are associated with pseudoxanthoma elasticum (PXE). Two transcripts encoding different isoforms have been described.
Genomic regions Chromosome 16
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NOMO2-212 ENST00000622306.4 4252 1222aa Protein coding CCDS10570 Q5JPE7-2 - TSL:1, GENCODE basic, APPRIS P4,
NOMO2-211 ENST00000621364.4 3921 1267aa Protein coding CCDS32394 Q5JPE7-1 - TSL:1, GENCODE basic, APPRIS ALT2,
NOMO2-201 ENST00000330537.10 4352 1222aa Protein coding - Q5JPE7-2 - TSL:5, GENCODE basic, APPRIS P4,
NOMO2-202 ENST00000381474.7 3911 1267aa Protein coding - Q5JPE7-1 - TSL:5, GENCODE basic, APPRIS ALT2,
NOMO2-203 ENST00000543392.5 3765 1100aa Protein coding - Q5JPE7-3 - TSL:1, GENCODE basic, APPRIS ALT2,
NOMO2-210 ENST00000620440.4 1360 282aa Protein coding - A0A087WTD5 - TSL:5, GENCODE basic,
NOMO2-206 ENST00000564991.5 3765 138aa Nonsense mediated decay - H3BTW1 - TSL:5,
NOMO2-208 ENST00000569051.1 901 223aa Nonsense mediated decay - H3BPS9 - CDS 5' incomplete, TSL:5,
NOMO2-204 ENST00000562312.1 682 61aa Nonsense mediated decay - H3BMJ6 - TSL:5,
NOMO2-207 ENST00000567831.5 551 80aa Nonsense mediated decay - H3BNC4 - CDS 5' incomplete, TSL:4,
NOMO2-205 ENST00000564355.1 592 No protein Retained intron - - - TSL:2,
NOMO2-209 ENST00000569526.1 545 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NOMO2
This KO Strategy loading
Red Cotton™ Notes Gene NOMO2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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