CRISPR-U™ Gene Knockout Cell Line
Strategy
NOMO2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NOMO2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NOMO2 |
| Gene id | 283820 |
| Organism | Homo sapiens |
| Official full symbol | NODAL modulator 2 |
| Gene type | protein-coding |
| Also known as | Nomo, PM5 |
| Summary | This gene encodes a protein originally thought to be related to the collagenase gene family. This gene is one of three highly similar genes in a region of duplication located on the p arm of chromosome 16. These three genes encode closely related proteins that may have the same function. The protein encoded by one of these genes has been identified as part of a protein complex that participates in the Nodal signaling pathway during vertebrate development. Mutations in ABCC6, which is located nearby, rather than mutations in this gene are associated with pseudoxanthoma elasticum (PXE). Two transcripts encoding different isoforms have been described. |
| Genomic regions | Chromosome 16 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NOMO2-212 | ENST00000622306.4 | 4252 | 1222aa | Protein coding | CCDS10570 | Q5JPE7-2 | - | TSL:1, GENCODE basic, APPRIS P4, |
| NOMO2-211 | ENST00000621364.4 | 3921 | 1267aa | Protein coding | CCDS32394 | Q5JPE7-1 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| NOMO2-201 | ENST00000330537.10 | 4352 | 1222aa | Protein coding | - | Q5JPE7-2 | - | TSL:5, GENCODE basic, APPRIS P4, |
| NOMO2-202 | ENST00000381474.7 | 3911 | 1267aa | Protein coding | - | Q5JPE7-1 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| NOMO2-203 | ENST00000543392.5 | 3765 | 1100aa | Protein coding | - | Q5JPE7-3 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| NOMO2-210 | ENST00000620440.4 | 1360 | 282aa | Protein coding | - | A0A087WTD5 | - | TSL:5, GENCODE basic, |
| NOMO2-206 | ENST00000564991.5 | 3765 | 138aa | Nonsense mediated decay | - | H3BTW1 | - | TSL:5, |
| NOMO2-208 | ENST00000569051.1 | 901 | 223aa | Nonsense mediated decay | - | H3BPS9 | - | CDS 5' incomplete, TSL:5, |
| NOMO2-204 | ENST00000562312.1 | 682 | 61aa | Nonsense mediated decay | - | H3BMJ6 | - | TSL:5, |
| NOMO2-207 | ENST00000567831.5 | 551 | 80aa | Nonsense mediated decay | - | H3BNC4 | - | CDS 5' incomplete, TSL:4, |
| NOMO2-205 | ENST00000564355.1 | 592 | No protein | Retained intron | - | - | - | TSL:2, |
| NOMO2-209 | ENST00000569526.1 | 545 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NOMO2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NOMO2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
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Work flow
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