CRISPR-U™ Gene Knockout Cell Line Strategy

GNS Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human GNS Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol GNS
Gene id 2799
Organism Homo sapiens
Official full symbol glucosamine (N-acetyl)-6-sulfatase
Gene type protein-coding
Also known as G6S
Summary The product of this gene is a lysosomal enzyme found in all cells. It is involved in the catabolism of heparin, heparan sulphate, and keratan sulphate. Deficiency of this enzyme results in the accumulation of undegraded substrate and the lysosomal storage disorder mucopolysaccharidosis type IIID (Sanfilippo D syndrome). Mucopolysaccharidosis type IIID is the least common of the four subtypes of Sanfilippo syndrome.
Genomic regions Chromosome 12
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
GNS-201 ENST00000258145.8 5081 552aa Protein coding CCDS8970 P15586-1 NM_002076.4 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
GNS-202 ENST00000418919.6 4877 496aa Protein coding - H7C3P4 - TSL:1, GENCODE basic,
GNS-208 ENST00000543646.5 2164 584aa Protein coding - F6S8M0 - TSL:2, GENCODE basic, APPRIS ALT2,
GNS-207 ENST00000542058.5 2020 532aa Protein coding - P15586-2 - TSL:2, GENCODE basic,
GNS-204 ENST00000540196.5 907 303aa Protein coding - H0YFA9 - CDS 5' and 3' incomplete, TSL:5,
GNS-209 ENST00000545273.1 906 247aa Protein coding - F5H4C6 - CDS 3' incomplete, TSL:5,
GNS-206 ENST00000541781.5 1846 No protein Retained intron - - - TSL:5,
GNS-203 ENST00000537823.1 562 No protein Retained intron - - - TSL:3,
GNS-205 ENST00000540883.1 544 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene GNS
This KO Strategy loading
Red Cotton™ Notes Gene GNS had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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