CRISPR-U™ Gene Knockout Cell Line Strategy

GNG5 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human GNG5 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol GNG5
Gene id 2787
Organism Homo sapiens
Official full symbol G protein subunit gamma 5
Gene type protein-coding
Summary G proteins are trimeric (alpha-beta-gamma) membrane-associated proteins that regulate flow of information from cell surface receptors to a variety of internal metabolic effectors. Interaction of a G protein with its activated receptor promotes exchange of GTP for GDP that is bound to the alpha subunit. The alpha-GTP complex dissociates from the beta-gamma heterodimer so that the subunits, in turn, may interact with and regulate effector molecules (Gilman, 1987 ; summary by Ahmad et al., 1995) .
Genomic regions Chromosome 1
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
GNG5-201 ENST00000370641.3 920 68aa Protein coding CCDS696 P63218 - TSL:1, GENCODE basic, APPRIS P1,
GNG5-202 ENST00000370645.9 806 68aa Protein coding CCDS696 P63218 NM_005274.3 TSL:2, GENCODE basic, APPRIS P1, MANE Select v0.92,
GNG5-203 ENST00000487806.5 557 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene GNG5
This KO Strategy loading
Red Cotton™ Notes Gene GNG5 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$49

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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