CRISPR-U™ Gene Knockout Cell Line
Strategy
GNG5 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human GNG5 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | GNG5 |
| Gene id | 2787 |
| Organism | Homo sapiens |
| Official full symbol | G protein subunit gamma 5 |
| Gene type | protein-coding |
| Summary | G proteins are trimeric (alpha-beta-gamma) membrane-associated proteins that regulate flow of information from cell surface receptors to a variety of internal metabolic effectors. Interaction of a G protein with its activated receptor promotes exchange of GTP for GDP that is bound to the alpha subunit. The alpha-GTP complex dissociates from the beta-gamma heterodimer so that the subunits, in turn, may interact with and regulate effector molecules (Gilman, 1987 ; summary by Ahmad et al., 1995) . |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| GNG5-201 | ENST00000370641.3 | 920 | 68aa | Protein coding | CCDS696 | P63218 | - | TSL:1, GENCODE basic, APPRIS P1, |
| GNG5-202 | ENST00000370645.9 | 806 | 68aa | Protein coding | CCDS696 | P63218 | NM_005274.3 | TSL:2, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| GNG5-203 | ENST00000487806.5 | 557 | No protein | Processed transcript | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | GNG5 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene GNG5 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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