CRISPR-U™ Gene Knockout Cell Line Strategy

GNB1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human GNB1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol GNB1
Gene id 2782
Organism Homo sapiens
Official full symbol G protein subunit beta 1
Gene type protein-coding
Also known as MRD42
Summary Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 1
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
GNB1-201 ENST00000378609.9 3163 340aa Protein coding CCDS34 P62873-1 NM_002074.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
GNB1-209 ENST00000610897.4 3145 340aa Protein coding CCDS34 P62873-1 - TSL:5, GENCODE basic, APPRIS P1,
GNB1-210 ENST00000615252.4 3048 240aa Protein coding CCDS72685 B3KVK2 - TSL:5, GENCODE basic,
GNB1-202 ENST00000434686.6 684 165aa Protein coding - F6UT28 - CDS 3' incomplete, TSL:3,
GNB1-204 ENST00000439272.6 602 163aa Protein coding - F6X3N5 - CDS 3' incomplete, TSL:5,
GNB1-203 ENST00000437146.1 423 107aa Protein coding - B1AKQ8 - CDS 3' incomplete, TSL:3,
GNB1-207 ENST00000472614.2 708 No protein Processed transcript - - - TSL:2,
GNB1-205 ENST00000461893.5 2438 No protein Retained intron - - - TSL:2,
GNB1-206 ENST00000471354.1 1512 No protein Retained intron - - - TSL:5,
GNB1-208 ENST00000607589.1 567 No protein Retained intron - - - TSL:NA,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene GNB1
This KO Strategy loading
Red Cotton™ Notes Gene GNB1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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