CRISPR-U™ Gene Knockout Cell Line
Strategy
GNB1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human GNB1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | GNB1 |
| Gene id | 2782 |
| Organism | Homo sapiens |
| Official full symbol | G protein subunit beta 1 |
| Gene type | protein-coding |
| Also known as | MRD42 |
| Summary | Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| GNB1-201 | ENST00000378609.9 | 3163 | 340aa | Protein coding | CCDS34 | P62873-1 | NM_002074.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| GNB1-209 | ENST00000610897.4 | 3145 | 340aa | Protein coding | CCDS34 | P62873-1 | - | TSL:5, GENCODE basic, APPRIS P1, |
| GNB1-210 | ENST00000615252.4 | 3048 | 240aa | Protein coding | CCDS72685 | B3KVK2 | - | TSL:5, GENCODE basic, |
| GNB1-202 | ENST00000434686.6 | 684 | 165aa | Protein coding | - | F6UT28 | - | CDS 3' incomplete, TSL:3, |
| GNB1-204 | ENST00000439272.6 | 602 | 163aa | Protein coding | - | F6X3N5 | - | CDS 3' incomplete, TSL:5, |
| GNB1-203 | ENST00000437146.1 | 423 | 107aa | Protein coding | - | B1AKQ8 | - | CDS 3' incomplete, TSL:3, |
| GNB1-207 | ENST00000472614.2 | 708 | No protein | Processed transcript | - | - | - | TSL:2, |
| GNB1-205 | ENST00000461893.5 | 2438 | No protein | Retained intron | - | - | - | TSL:2, |
| GNB1-206 | ENST00000471354.1 | 1512 | No protein | Retained intron | - | - | - | TSL:5, |
| GNB1-208 | ENST00000607589.1 | 567 | No protein | Retained intron | - | - | - | TSL:NA, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | GNB1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene GNB1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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