CRISPR-U™ Gene Knockout Cell Line
Strategy
GMPR Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human GMPR Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | GMPR |
| Gene id | 2766 |
| Organism | Homo sapiens |
| Official full symbol | guanosine monophosphate reductase |
| Gene type | protein-coding |
| Also known as | GMPR 1, GMPR1 |
| Summary | This gene encodes an enzyme that catalyzes the irreversible and NADPH-dependent reductive deamination of GMP to IMP. The protein also functions in the re-utilization of free intracellular bases and purine nucleosides. |
| Genomic regions | Chromosome 6 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| GMPR-201 | ENST00000259727.5 | 1508 | 345aa | Protein coding | CCDS4537 | P36959 | NM_006877.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| GMPR-204 | ENST00000544145.1 | 750 | No protein | Processed transcript | - | - | - | TSL:3, |
| GMPR-203 | ENST00000543191.5 | 675 | No protein | Processed transcript | - | - | - | TSL:2, |
| GMPR-202 | ENST00000540478.1 | 793 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | GMPR |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene GMPR had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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