CRISPR-U™ Gene Knockout Cell Line
Strategy
GLUL Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human GLUL Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | GLUL |
| Gene id | 2752 |
| Organism | Homo sapiens |
| Official full symbol | glutamate-ammonia ligase |
| Gene type | protein-coding |
| Also known as | GLNS, GS, PIG43, PIG59 |
| Summary | The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| GLUL-202 | ENST00000331872.11 | 7554 | 373aa | Protein coding | CCDS1344 | A8YXX4 P15104 | NM_001033044.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| GLUL-201 | ENST00000311223.9 | 4719 | 373aa | Protein coding | CCDS1344 | A8YXX4 P15104 | - | TSL:1, GENCODE basic, APPRIS P1, |
| GLUL-204 | ENST00000417584.6 | 4065 | 373aa | Protein coding | CCDS1344 | A8YXX4 P15104 | - | TSL:5, GENCODE basic, APPRIS P1, |
| GLUL-203 | ENST00000339526.8 | 3942 | 373aa | Protein coding | CCDS1344 | A8YXX4 P15104 | - | TSL:1, GENCODE basic, APPRIS P1, |
| GLUL-213 | ENST00000642379.1 | 4364 | 507aa | Protein coding | - | A0A2R8YDT1 | - | GENCODE basic, |
| GLUL-212 | ENST00000491322.1 | 6132 | No protein | Processed transcript | - | - | - | TSL:1, |
| GLUL-210 | ENST00000484996.5 | 1369 | No protein | Processed transcript | - | - | - | TSL:3, |
| GLUL-207 | ENST00000463851.6 | 939 | No protein | Processed transcript | - | - | - | TSL:3, |
| GLUL-209 | ENST00000480604.5 | 908 | No protein | Processed transcript | - | - | - | TSL:5, |
| GLUL-208 | ENST00000475808.5 | 868 | No protein | Processed transcript | - | - | - | TSL:2, |
| GLUL-206 | ENST00000462444.1 | 793 | No protein | Processed transcript | - | - | - | TSL:2, |
| GLUL-205 | ENST00000461447.1 | 689 | No protein | Processed transcript | - | - | - | TSL:2, |
| GLUL-211 | ENST00000489818.5 | 683 | No protein | Processed transcript | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | GLUL |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene GLUL had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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