CRISPR-U™ Gene Knockout Cell Line
Strategy
EML4 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human EML4 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | EML4 |
| Gene id | 27436 |
| Organism | Homo sapiens |
| Official full symbol | EMAP like 4 |
| Gene type | protein-coding |
| Also known as | C2orf2, ELP120, EMAP-4, EMAPL4, ROPP120 |
| Summary | This gene is a member of the echinoderm microtubule associated protein-like family. The encoded WD-repeat protein may be involved in microtubule formation. Abnormal fusion of parts of this gene with portions of the anaplastic lymphoma receptor tyrosine kinase gene, which generates EML4-ALK fusion transcripts, is one of the primary mutations associated with non-small cell lung cancer. Alternative splicing of this gene results in two transcript variants. |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| EML4-201 | ENST00000318522.10 | 5546 | 981aa | Protein coding | CCDS1807 | Q9HC35-1 | NM_019063.5 | TSL:1, GENCODE basic, APPRIS P3, MANE Select v0.92, |
| EML4-203 | ENST00000402711.6 | 3568 | 923aa | Protein coding | CCDS46266 | Q9HC35-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| EML4-202 | ENST00000401738.3 | 3797 | 992aa | Protein coding | - | B5MBZ0 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| EML4-208 | ENST00000482660.1 | 584 | No protein | Processed transcript | - | - | - | TSL:4, |
| EML4-206 | ENST00000472632.1 | 515 | No protein | Processed transcript | - | - | - | TSL:3, |
| EML4-204 | ENST00000406175.3 | 4622 | No protein | Retained intron | - | - | - | TSL:1, |
| EML4-207 | ENST00000480320.1 | 1932 | No protein | Retained intron | - | - | - | TSL:2, |
| EML4-205 | ENST00000409040.1 | 962 | No protein | Retained intron | - | - | - | TSL:1, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | EML4 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene EML4 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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