CRISPR-U™ Gene Knockout Cell Line
Strategy
POLM Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human POLM Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | POLM |
| Gene id | 27434 |
| Organism | Homo sapiens |
| Official full symbol | DNA polymerase mu |
| Gene type | protein-coding |
| Also known as | Pol Mu, Tdt-N |
| Genomic regions | Chromosome 7 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| POLM-201 | ENST00000242248.10 | 2806 | 494aa | Protein coding | CCDS34625 | Q9NP87-1 | NM_013284.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| POLM-202 | ENST00000335195.10 | 2444 | 457aa | Protein coding | CCDS64635 | Q9NP87-2 | - | TSL:1, GENCODE basic, |
| POLM-203 | ENST00000395831.7 | 2380 | 508aa | Protein coding | CCDS64636 | Q9NP87-3 | - | TSL:1, GENCODE basic, |
| POLM-210 | ENST00000452049.1 | 587 | 165aa | Protein coding | - | C9J222 | - | CDS 3' incomplete, TSL:3, |
| POLM-204 | ENST00000414235.5 | 550 | 159aa | Protein coding | - | C9JF34 | - | CDS 3' incomplete, TSL:2, |
| POLM-211 | ENST00000458246.5 | 3218 | 270aa | Nonsense mediated decay | - | Q6PIY2 | - | TSL:1, |
| POLM-206 | ENST00000430942.5 | 2561 | 242aa | Nonsense mediated decay | - | Q9H980 | - | TSL:2, |
| POLM-208 | ENST00000435068.5 | 2404 | 155aa | Nonsense mediated decay | - | F8WEJ1 | - | TSL:2, |
| POLM-207 | ENST00000434229.5 | 1174 | 233aa | Nonsense mediated decay | - | F8WDE4 | - | TSL:1, |
| POLM-209 | ENST00000445616.5 | 811 | 168aa | Nonsense mediated decay | - | F8WB78 | - | TSL:3, |
| POLM-205 | ENST00000418926.1 | 546 | 135aa | Nonsense mediated decay | - | H7C2M2 | - | CDS 5' incomplete, TSL:4, |
| POLM-217 | ENST00000492971.1 | 492 | No protein | Processed transcript | - | - | - | TSL:5, |
| POLM-216 | ENST00000492605.5 | 1649 | No protein | Retained intron | - | - | - | TSL:1, |
| POLM-214 | ENST00000492312.5 | 1149 | No protein | Retained intron | - | - | - | TSL:1, |
| POLM-215 | ENST00000492321.5 | 962 | No protein | Retained intron | - | - | - | TSL:2, |
| POLM-212 | ENST00000467607.1 | 580 | No protein | Retained intron | - | - | - | TSL:2, |
| POLM-218 | ENST00000497300.5 | 575 | No protein | Retained intron | - | - | - | TSL:4, |
| POLM-213 | ENST00000483644.1 | 561 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | POLM |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene POLM had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: