CRISPR-U™ Gene Knockout Cell Line Strategy

POLM Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human POLM Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol POLM
Gene id 27434
Organism Homo sapiens
Official full symbol DNA polymerase mu
Gene type protein-coding
Also known as Pol Mu, Tdt-N
Genomic regions Chromosome 7
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
POLM-201 ENST00000242248.10 2806 494aa Protein coding CCDS34625 Q9NP87-1 NM_013284.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
POLM-202 ENST00000335195.10 2444 457aa Protein coding CCDS64635 Q9NP87-2 - TSL:1, GENCODE basic,
POLM-203 ENST00000395831.7 2380 508aa Protein coding CCDS64636 Q9NP87-3 - TSL:1, GENCODE basic,
POLM-210 ENST00000452049.1 587 165aa Protein coding - C9J222 - CDS 3' incomplete, TSL:3,
POLM-204 ENST00000414235.5 550 159aa Protein coding - C9JF34 - CDS 3' incomplete, TSL:2,
POLM-211 ENST00000458246.5 3218 270aa Nonsense mediated decay - Q6PIY2 - TSL:1,
POLM-206 ENST00000430942.5 2561 242aa Nonsense mediated decay - Q9H980 - TSL:2,
POLM-208 ENST00000435068.5 2404 155aa Nonsense mediated decay - F8WEJ1 - TSL:2,
POLM-207 ENST00000434229.5 1174 233aa Nonsense mediated decay - F8WDE4 - TSL:1,
POLM-209 ENST00000445616.5 811 168aa Nonsense mediated decay - F8WB78 - TSL:3,
POLM-205 ENST00000418926.1 546 135aa Nonsense mediated decay - H7C2M2 - CDS 5' incomplete, TSL:4,
POLM-217 ENST00000492971.1 492 No protein Processed transcript - - - TSL:5,
POLM-216 ENST00000492605.5 1649 No protein Retained intron - - - TSL:1,
POLM-214 ENST00000492312.5 1149 No protein Retained intron - - - TSL:1,
POLM-215 ENST00000492321.5 962 No protein Retained intron - - - TSL:2,
POLM-212 ENST00000467607.1 580 No protein Retained intron - - - TSL:2,
POLM-218 ENST00000497300.5 575 No protein Retained intron - - - TSL:4,
POLM-213 ENST00000483644.1 561 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene POLM
This KO Strategy loading
Red Cotton™ Notes Gene POLM had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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