CRISPR-U™ Gene Knockout Cell Line Strategy

MAT2B Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MAT2B Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MAT2B
Gene id 27430
Organism Homo sapiens
Official full symbol methionine adenosyltransferase 2B
Gene type protein-coding
Also known as MAT-II, MATIIbeta, Nbla02999, SDR23E1, TGR
Summary The protein encoded by this gene belongs to the methionine adenosyltransferase (MAT) family. MAT catalyzes the biosynthesis of S-adenosylmethionine from methionine and ATP. This protein is the regulatory beta subunit of MAT. Alternative splicing results in multiple transcript variants encoding different isoforms.
Genomic regions Chromosome 5
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MAT2B-201 ENST00000280969.9 2226 323aa Protein coding CCDS4364 Q9NZL9-2 - TSL:1, GENCODE basic,
MAT2B-202 ENST00000321757.11 2064 334aa Protein coding CCDS4365 Q9NZL9-1 NM_013283.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
MAT2B-204 ENST00000518095.5 3409 259aa Protein coding - Q9NZL9-3 - TSL:1, GENCODE basic,
MAT2B-203 ENST00000421814.6 698 198aa Protein coding - H7C0X7 - CDS 3' incomplete, TSL:5,
MAT2B-206 ENST00000520449.1 557 117aa Nonsense mediated decay - E5RJR3 - TSL:4,
MAT2B-207 ENST00000521838.2 1526 No protein Processed transcript - - - TSL:5,
MAT2B-208 ENST00000523606.1 3210 No protein Retained intron - - - TSL:2,
MAT2B-205 ENST00000519719.1 572 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MAT2B
This KO Strategy loading
Red Cotton™ Notes Gene MAT2B had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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