CRISPR-U™ Gene Knockout Cell Line
Strategy
MAT2B Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MAT2B Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MAT2B |
| Gene id | 27430 |
| Organism | Homo sapiens |
| Official full symbol | methionine adenosyltransferase 2B |
| Gene type | protein-coding |
| Also known as | MAT-II, MATIIbeta, Nbla02999, SDR23E1, TGR |
| Summary | The protein encoded by this gene belongs to the methionine adenosyltransferase (MAT) family. MAT catalyzes the biosynthesis of S-adenosylmethionine from methionine and ATP. This protein is the regulatory beta subunit of MAT. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| Genomic regions | Chromosome 5 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MAT2B-201 | ENST00000280969.9 | 2226 | 323aa | Protein coding | CCDS4364 | Q9NZL9-2 | - | TSL:1, GENCODE basic, |
| MAT2B-202 | ENST00000321757.11 | 2064 | 334aa | Protein coding | CCDS4365 | Q9NZL9-1 | NM_013283.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| MAT2B-204 | ENST00000518095.5 | 3409 | 259aa | Protein coding | - | Q9NZL9-3 | - | TSL:1, GENCODE basic, |
| MAT2B-203 | ENST00000421814.6 | 698 | 198aa | Protein coding | - | H7C0X7 | - | CDS 3' incomplete, TSL:5, |
| MAT2B-206 | ENST00000520449.1 | 557 | 117aa | Nonsense mediated decay | - | E5RJR3 | - | TSL:4, |
| MAT2B-207 | ENST00000521838.2 | 1526 | No protein | Processed transcript | - | - | - | TSL:5, |
| MAT2B-208 | ENST00000523606.1 | 3210 | No protein | Retained intron | - | - | - | TSL:2, |
| MAT2B-205 | ENST00000519719.1 | 572 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MAT2B |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MAT2B had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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