CRISPR-U™ Gene Knockout Cell Line
Strategy
GLI4 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human GLI4 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | GLI4 |
| Gene id | 2738 |
| Organism | Homo sapiens |
| Official full symbol | GLI family zinc finger 4 |
| Gene type | protein-coding |
| Also known as | HKR4, ZNF928 |
| Genomic regions | Chromosome 8 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| GLI4-201 | ENST00000340042.2 | 1335 | 376aa | Protein coding | CCDS6398 | P10075 | NM_138465.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| GLI4-210 | ENST00000523522.5 | 1297 | 376aa | Protein coding | CCDS6398 | P10075 | - | TSL:1, GENCODE basic, APPRIS P1, |
| GLI4-207 | ENST00000521682.5 | 1082 | 165aa | Protein coding | - | E5RG07 | - | TSL:3, GENCODE basic, |
| GLI4-202 | ENST00000344692.7 | 1012 | 76aa | Protein coding | - | E5RGM1 | - | TSL:2, GENCODE basic, |
| GLI4-203 | ENST00000517468.5 | 963 | 56aa | Protein coding | - | E5RIX4 | - | TSL:5, GENCODE basic, |
| GLI4-204 | ENST00000517530.5 | 563 | 86aa | Protein coding | - | H0YBH2 | - | CDS 5' incomplete, TSL:2, |
| GLI4-206 | ENST00000520021.5 | 361 | 111aa | Protein coding | - | E5RFY1 | - | CDS 3' incomplete, TSL:3, |
| GLI4-211 | ENST00000523812.1 | 2333 | No protein | Processed transcript | - | - | - | TSL:NA, |
| GLI4-205 | ENST00000519876.1 | 585 | No protein | Processed transcript | - | - | - | TSL:3, |
| GLI4-209 | ENST00000522479.1 | 435 | No protein | Processed transcript | - | - | - | TSL:3, |
| GLI4-208 | ENST00000522033.1 | 615 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | GLI4 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene GLI4 had been KO in hct116 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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