CRISPR-U™ Gene Knockout Cell Line Strategy

GLI4 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human GLI4 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol GLI4
Gene id 2738
Organism Homo sapiens
Official full symbol GLI family zinc finger 4
Gene type protein-coding
Also known as HKR4, ZNF928
Genomic regions Chromosome 8
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
GLI4-201 ENST00000340042.2 1335 376aa Protein coding CCDS6398 P10075 NM_138465.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
GLI4-210 ENST00000523522.5 1297 376aa Protein coding CCDS6398 P10075 - TSL:1, GENCODE basic, APPRIS P1,
GLI4-207 ENST00000521682.5 1082 165aa Protein coding - E5RG07 - TSL:3, GENCODE basic,
GLI4-202 ENST00000344692.7 1012 76aa Protein coding - E5RGM1 - TSL:2, GENCODE basic,
GLI4-203 ENST00000517468.5 963 56aa Protein coding - E5RIX4 - TSL:5, GENCODE basic,
GLI4-204 ENST00000517530.5 563 86aa Protein coding - H0YBH2 - CDS 5' incomplete, TSL:2,
GLI4-206 ENST00000520021.5 361 111aa Protein coding - E5RFY1 - CDS 3' incomplete, TSL:3,
GLI4-211 ENST00000523812.1 2333 No protein Processed transcript - - - TSL:NA,
GLI4-205 ENST00000519876.1 585 No protein Processed transcript - - - TSL:3,
GLI4-209 ENST00000522479.1 435 No protein Processed transcript - - - TSL:3,
GLI4-208 ENST00000522033.1 615 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene GLI4
This KO Strategy loading
Red Cotton™ Notes Gene GLI4 had been KO in hct116 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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