CRISPR-U™ Gene Knockout Cell Line Strategy

GOLIM4 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human GOLIM4 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol GOLIM4
Gene id 27333
Organism Homo sapiens
Official full symbol golgi integral membrane protein 4
Gene type protein-coding
Also known as GIMPC, GOLPH4, GPP130, P138
Summary The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi-resident protein. It may process proteins synthesized in the rough endoplasmic reticulum and assist in the transport of protein cargo through the Golgi apparatus.
Genomic regions Chromosome 3
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
GOLIM4-202 ENST00000470487.6 4310 696aa Protein coding CCDS3204 O00461 NM_014498.5 TSL:1, GENCODE basic, APPRIS P3, MANE Select v0.92,
GOLIM4-201 ENST00000309027.4 2100 668aa Protein coding CCDS77854 F8W785 - TSL:1, GENCODE basic, APPRIS ALT2,
GOLIM4-203 ENST00000479415.1 562 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene GOLIM4
This KO Strategy loading
Red Cotton™ Notes Gene GOLIM4 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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