CRISPR-U™ Gene Knockout Cell Line Strategy

PDCD4 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PDCD4 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PDCD4
Gene id 27250
Organism Homo sapiens
Official full symbol programmed cell death 4
Gene type protein-coding
Also known as H731
Summary This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 10
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PDCD4-202 ENST00000393104.6 3697 458aa Protein coding CCDS44478 Q53EL6-2 - TSL:1, GENCODE basic,
PDCD4-201 ENST00000280154.12 3481 469aa Protein coding CCDS7567 Q53EL6-1 NM_014456.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PDCD4-203 ENST00000444997.1 819 231aa Protein coding - Q5VZS7 - CDS 3' incomplete, TSL:3,
PDCD4-206 ENST00000481353.5 1120 No protein Processed transcript - - - TSL:2,
PDCD4-211 ENST00000498367.1 975 No protein Processed transcript - - - TSL:3,
PDCD4-210 ENST00000492932.5 813 No protein Processed transcript - - - TSL:3,
PDCD4-204 ENST00000462577.5 789 No protein Processed transcript - - - TSL:5,
PDCD4-208 ENST00000483670.5 774 No protein Processed transcript - - - TSL:5,
PDCD4-205 ENST00000467574.5 735 No protein Processed transcript - - - TSL:3,
PDCD4-209 ENST00000489049.5 547 No protein Processed transcript - - - TSL:2,
PDCD4-207 ENST00000483595.1 531 No protein Processed transcript - - - TSL:5,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PDCD4
This KO Strategy loading
Red Cotton™ Notes Gene PDCD4 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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