CRISPR-U™ Gene Knockout Cell Line
Strategy
PDCD4 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PDCD4 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PDCD4 |
| Gene id | 27250 |
| Organism | Homo sapiens |
| Official full symbol | programmed cell death 4 |
| Gene type | protein-coding |
| Also known as | H731 |
| Summary | This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 10 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PDCD4-202 | ENST00000393104.6 | 3697 | 458aa | Protein coding | CCDS44478 | Q53EL6-2 | - | TSL:1, GENCODE basic, |
| PDCD4-201 | ENST00000280154.12 | 3481 | 469aa | Protein coding | CCDS7567 | Q53EL6-1 | NM_014456.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PDCD4-203 | ENST00000444997.1 | 819 | 231aa | Protein coding | - | Q5VZS7 | - | CDS 3' incomplete, TSL:3, |
| PDCD4-206 | ENST00000481353.5 | 1120 | No protein | Processed transcript | - | - | - | TSL:2, |
| PDCD4-211 | ENST00000498367.1 | 975 | No protein | Processed transcript | - | - | - | TSL:3, |
| PDCD4-210 | ENST00000492932.5 | 813 | No protein | Processed transcript | - | - | - | TSL:3, |
| PDCD4-204 | ENST00000462577.5 | 789 | No protein | Processed transcript | - | - | - | TSL:5, |
| PDCD4-208 | ENST00000483670.5 | 774 | No protein | Processed transcript | - | - | - | TSL:5, |
| PDCD4-205 | ENST00000467574.5 | 735 | No protein | Processed transcript | - | - | - | TSL:3, |
| PDCD4-209 | ENST00000489049.5 | 547 | No protein | Processed transcript | - | - | - | TSL:2, |
| PDCD4-207 | ENST00000483595.1 | 531 | No protein | Processed transcript | - | - | - | TSL:5, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PDCD4 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PDCD4 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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