CRISPR-U™ Gene Knockout Cell Line
Strategy
ARFIP1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ARFIP1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ARFIP1 |
| Gene id | 27236 |
| Organism | Homo sapiens |
| Official full symbol | ADP ribosylation factor interacting protein 1 |
| Gene type | protein-coding |
| Also known as | HSU52521 |
| Genomic regions | Chromosome 4 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ARFIP1-201 | ENST00000353617.7 | 3542 | 373aa | Protein coding | CCDS34080 | P53367-1 | NM_001025595.3 | TSL:5, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| ARFIP1-205 | ENST00000451320.6 | 3424 | 373aa | Protein coding | CCDS34080 | P53367-1 | - | TSL:1, GENCODE basic, APPRIS P4, |
| ARFIP1-203 | ENST00000405727.6 | 1601 | 341aa | Protein coding | CCDS3780 | P53367-2 | - | TSL:1, GENCODE basic, APPRIS ALT1, |
| ARFIP1-202 | ENST00000356064.3 | 1302 | 341aa | Protein coding | CCDS3780 | P53367-2 | - | TSL:1, GENCODE basic, APPRIS ALT1, |
| ARFIP1-204 | ENST00000429148.6 | 1599 | 193aa | Protein coding | - | P53367-3 | - | TSL:2, GENCODE basic, |
| ARFIP1-209 | ENST00000511289.5 | 593 | No protein | Processed transcript | - | - | - | TSL:4, |
| ARFIP1-207 | ENST00000510497.1 | 571 | No protein | Processed transcript | - | - | - | TSL:4, |
| ARFIP1-211 | ENST00000514499.1 | 562 | No protein | Processed transcript | - | - | - | TSL:4, |
| ARFIP1-210 | ENST00000513361.1 | 547 | No protein | Processed transcript | - | - | - | TSL:4, |
| ARFIP1-206 | ENST00000509413.1 | 531 | No protein | Processed transcript | - | - | - | TSL:3, |
| ARFIP1-208 | ENST00000510831.5 | 514 | No protein | Processed transcript | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ARFIP1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ARFIP1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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