CRISPR-U™ Gene Knockout Cell Line Strategy

INVS Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human INVS Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol INVS
Gene id 27130
Organism Homo sapiens
Official full symbol inversin
Gene type protein-coding
Also known as INV, NPH2, NPHP2
Summary This gene encodes a protein containing multiple ankyrin domains and two IQ calmodulin-binding domains. The encoded protein may function in renal tubular development and function, and in left-right axis determination. This protein interacts with nephrocystin and infers a connection between primary cilia function and left-right axis determination. A similar protein in mice interacts with calmodulin. Mutations in this gene have been associated with nephronophthisis type 2. Multiple transcript variants encoding distinct isoforms have been identified for this gene.
Genomic regions Chromosome 9
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
INVS-202 ENST00000262457.7 4897 1065aa Protein coding CCDS6746 Q9Y283-1 NM_014425.5 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
INVS-201 ENST00000262456.6 2968 895aa Protein coding - Q9Y283-2 - TSL:5, GENCODE basic, APPRIS ALT2,
INVS-203 ENST00000374921.3 1654 101aa Protein coding - Q9Y283-3 - TSL:1, GENCODE basic,
INVS-204 ENST00000460636.2 954 No protein Processed transcript - - - TSL:5,
INVS-206 ENST00000480309.1 862 No protein Processed transcript - - - TSL:3,
INVS-205 ENST00000466647.5 767 No protein Processed transcript - - - TSL:3,
INVS-207 ENST00000496467.5 714 No protein Processed transcript - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene INVS
This KO Strategy loading
Red Cotton™ Notes Gene INVS had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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