CRISPR-U™ Gene Knockout Cell Line
Strategy
INVS Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human INVS Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | INVS |
| Gene id | 27130 |
| Organism | Homo sapiens |
| Official full symbol | inversin |
| Gene type | protein-coding |
| Also known as | INV, NPH2, NPHP2 |
| Summary | This gene encodes a protein containing multiple ankyrin domains and two IQ calmodulin-binding domains. The encoded protein may function in renal tubular development and function, and in left-right axis determination. This protein interacts with nephrocystin and infers a connection between primary cilia function and left-right axis determination. A similar protein in mice interacts with calmodulin. Mutations in this gene have been associated with nephronophthisis type 2. Multiple transcript variants encoding distinct isoforms have been identified for this gene. |
| Genomic regions | Chromosome 9 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| INVS-202 | ENST00000262457.7 | 4897 | 1065aa | Protein coding | CCDS6746 | Q9Y283-1 | NM_014425.5 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| INVS-201 | ENST00000262456.6 | 2968 | 895aa | Protein coding | - | Q9Y283-2 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| INVS-203 | ENST00000374921.3 | 1654 | 101aa | Protein coding | - | Q9Y283-3 | - | TSL:1, GENCODE basic, |
| INVS-204 | ENST00000460636.2 | 954 | No protein | Processed transcript | - | - | - | TSL:5, |
| INVS-206 | ENST00000480309.1 | 862 | No protein | Processed transcript | - | - | - | TSL:3, |
| INVS-205 | ENST00000466647.5 | 767 | No protein | Processed transcript | - | - | - | TSL:3, |
| INVS-207 | ENST00000496467.5 | 714 | No protein | Processed transcript | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | INVS |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene INVS had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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