CRISPR-U™ Gene Knockout Cell Line Strategy

BBC3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human BBC3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol BBC3
Gene id 27113
Organism Homo sapiens
Official full symbol BCL2 binding component 3
Gene type protein-coding
Also known as JFY-1, JFY1, PUMA
Summary This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 19
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
BBC3-203 ENST00000439096.3 1846 193aa Protein coding CCDS12697 Q9BXH1-1 NM_014417.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
BBC3-204 ENST00000449228.5 1827 261aa Protein coding CCDS46129 Q96PG8-2 - TSL:1, GENCODE basic,
BBC3-202 ENST00000341983.8 1538 131aa Protein coding CCDS46130 Q9BXH1-2 - TSL:1, GENCODE basic,
BBC3-201 ENST00000300880.11 1347 101aa Protein coding CCDS46128 Q96PG8-1 - TSL:1, GENCODE basic,
BBC3-206 ENST00000601438.2 1747 No protein Retained intron - - - TSL:NA,
BBC3-205 ENST00000598636.1 900 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene BBC3
This KO Strategy loading
Red Cotton™ Notes Gene BBC3 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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