CRISPR-U™ Gene Knockout Cell Line
Strategy
BBC3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human BBC3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | BBC3 |
| Gene id | 27113 |
| Organism | Homo sapiens |
| Official full symbol | BCL2 binding component 3 |
| Gene type | protein-coding |
| Also known as | JFY-1, JFY1, PUMA |
| Summary | This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| BBC3-203 | ENST00000439096.3 | 1846 | 193aa | Protein coding | CCDS12697 | Q9BXH1-1 | NM_014417.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| BBC3-204 | ENST00000449228.5 | 1827 | 261aa | Protein coding | CCDS46129 | Q96PG8-2 | - | TSL:1, GENCODE basic, |
| BBC3-202 | ENST00000341983.8 | 1538 | 131aa | Protein coding | CCDS46130 | Q9BXH1-2 | - | TSL:1, GENCODE basic, |
| BBC3-201 | ENST00000300880.11 | 1347 | 101aa | Protein coding | CCDS46128 | Q96PG8-1 | - | TSL:1, GENCODE basic, |
| BBC3-206 | ENST00000601438.2 | 1747 | No protein | Retained intron | - | - | - | TSL:NA, |
| BBC3-205 | ENST00000598636.1 | 900 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | BBC3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene BBC3 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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