CRISPR-U™ Gene Knockout Cell Line
Strategy
LAMP3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human LAMP3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | LAMP3 |
| Gene id | 27074 |
| Organism | Homo sapiens |
| Official full symbol | lysosomal associated membrane protein 3 |
| Gene type | protein-coding |
| Also known as | CD208, DC LAMP, DC-LAMP, DCLAMP, LAMP, LAMP-3, TSC403 |
| Summary | Dendritic cells (DCs) are the most potent antigen-presenting cells. Immature DCs efficiently capture antigens and differentiate into interdigitating dendritic cells (IDCs) in lymphoid tissues that induce primary T-cell responses (summary by de Saint-Vis et al., 1998 ). |
| Genomic regions | Chromosome 3 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| LAMP3-201 | ENST00000265598.8 | 3196 | 416aa | Protein coding | CCDS3242 | Q9UQV4 | NM_014398.4 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| LAMP3-202 | ENST00000466939.1 | 1453 | 392aa | Protein coding | - | E7ETP9 | - | TSL:2, GENCODE basic, APPRIS ALT2, |
| LAMP3-204 | ENST00000476015.1 | 835 | 180aa | Protein coding | - | C9JYP5 | - | CDS 3' incomplete, TSL:4, |
| LAMP3-203 | ENST00000470251.1 | 829 | 143aa | Protein coding | - | C9JDI8 | - | CDS 3' incomplete, TSL:2, |
| LAMP3-205 | ENST00000486686.1 | 469 | No protein | Processed transcript | - | - | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | LAMP3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene LAMP3 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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