CRISPR-U™ Gene Knockout Cell Line Strategy

GHITM Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human GHITM Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol GHITM
Gene id 27069
Organism Homo sapiens
Official full symbol growth hormone inducible transmembrane protein
Gene type protein-coding
Also known as DERP2, HSPC282, MICS1, My021, PTD010, TMBIM5
Genomic regions Chromosome 10
Strategy Summary
This gene has 1 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
GHITM-201 ENST00000372134.6 2382 345aa Protein coding CCDS41542 Q9H3K2 NM_014394.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene GHITM
This KO Strategy loading
Red Cotton™ Notes Gene GHITM had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$49

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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