CRISPR-U™ Gene Knockout Cell Line
Strategy
TRMT2A Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human TRMT2A Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | TRMT2A |
| Gene id | 27037 |
| Organism | Homo sapiens |
| Official full symbol | tRNA methyltransferase 2 homolog A |
| Gene type | protein-coding |
| Also known as | HTF9C |
| Summary | The protein encoded by this gene is of unknown function. However, it is orthologous to the mouse Trmt2a gene and contains an RNA methyltransferase domain. Expression of this gene varies during the cell cycle, with aberrant expression being a possible biomarker in certain breast cancers. Several transcript variants encoding two different isoforms have been found for this gene. |
| Genomic regions | Chromosome 22 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| TRMT2A-202 | ENST00000403707.7 | 2928 | 625aa | Protein coding | CCDS13774 | Q8IZ69-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| TRMT2A-201 | ENST00000252136.12 | 2886 | 625aa | Protein coding | CCDS13774 | Q8IZ69-1 | NM_022727.6 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| TRMT2A-203 | ENST00000404751.7 | 2498 | 562aa | Protein coding | CCDS58793 | Q8IZ69-2 | - | TSL:2, GENCODE basic, |
| TRMT2A-204 | ENST00000439169.2 | 2473 | 643aa | Protein coding | CCDS82693 | F2Z2W7 | - | TSL:5, GENCODE basic, |
| TRMT2A-206 | ENST00000444845.5 | 739 | 158aa | Protein coding | - | H7C100 | - | CDS 5' incomplete, TSL:3, |
| TRMT2A-207 | ENST00000445045.1 | 582 | 119aa | Protein coding | - | C9K041 | - | CDS 3' incomplete, TSL:4, |
| TRMT2A-205 | ENST00000444256.1 | 426 | 142aa | Protein coding | - | H7BZZ4 | - | CDS 5' and 3' incomplete, TSL:5, |
| TRMT2A-218 | ENST00000492988.5 | 854 | No protein | Processed transcript | - | - | - | TSL:5, |
| TRMT2A-217 | ENST00000488335.1 | 379 | No protein | Processed transcript | - | - | - | TSL:3, |
| TRMT2A-214 | ENST00000480460.1 | 378 | No protein | Processed transcript | - | - | - | TSL:3, |
| TRMT2A-220 | ENST00000494820.5 | 1098 | No protein | Retained intron | - | - | - | TSL:5, |
| TRMT2A-219 | ENST00000494641.1 | 1060 | No protein | Retained intron | - | - | - | TSL:3, |
| TRMT2A-216 | ENST00000487668.5 | 1040 | No protein | Retained intron | - | - | - | TSL:5, |
| TRMT2A-211 | ENST00000468917.1 | 957 | No protein | Retained intron | - | - | - | TSL:2, |
| TRMT2A-210 | ENST00000464535.1 | 943 | No protein | Retained intron | - | - | - | TSL:2, |
| TRMT2A-212 | ENST00000471040.5 | 848 | No protein | Retained intron | - | - | - | TSL:3, |
| TRMT2A-213 | ENST00000480339.1 | 762 | No protein | Retained intron | - | - | - | TSL:2, |
| TRMT2A-209 | ENST00000463710.1 | 757 | No protein | Retained intron | - | - | - | TSL:3, |
| TRMT2A-215 | ENST00000487378.1 | 654 | No protein | Retained intron | - | - | - | TSL:2, |
| TRMT2A-208 | ENST00000459644.1 | 582 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | TRMT2A |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene TRMT2A had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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