CRISPR-U™ Gene Knockout Cell Line Strategy

ATP2C1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ATP2C1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ATP2C1
Gene id 27032
Organism Homo sapiens
Official full symbol ATPase secretory pathway Ca2+ transporting 1
Gene type protein-coding
Also known as ATP2C1A, BCPM, HHD, PMR1, SPCA1, hSPCA1
Summary The protein encoded by this gene belongs to the family of P-type cation transport ATPases. This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium ions. Defects in this gene cause Hailey-Hailey disease, an autosomal dominant disorder. Alternatively spliced transcript variants encoding different isoforms have been identified.
Genomic regions Chromosome 3
Strategy Summary
This gene has 18 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ATP2C1-218 ENST00000510168.6 4994 919aa Protein coding CCDS46914 P98194-1 NM_001378687.1 TSL:5, GENCODE basic, APPRIS ALT1, MANE Select v0.92,
ATP2C1-214 ENST00000508532.5 4969 919aa Protein coding CCDS46914 P98194-1 - TSL:1, GENCODE basic, APPRIS ALT1,
ATP2C1-210 ENST00000505330.5 4888 953aa Protein coding CCDS75006 B4E2Q0 - TSL:2, GENCODE basic,
ATP2C1-204 ENST00000428331.6 4795 919aa Protein coding CCDS46914 P98194-1 - TSL:1, GENCODE basic, APPRIS ALT1,
ATP2C1-208 ENST00000504948.5 4714 903aa Protein coding CCDS56281 P98194-4 - TSL:1, GENCODE basic, APPRIS P4,
ATP2C1-224 ENST00000533801.6 3690 888aa Protein coding CCDS46913 P98194-2 - TSL:1, GENCODE basic,
ATP2C1-212 ENST00000507488.6 3579 973aa Protein coding CCDS56278 P98194-7 - TSL:2, GENCODE basic,
ATP2C1-205 ENST00000504381.5 3498 944aa Protein coding CCDS56279 P98194-8 - TSL:2, GENCODE basic,
ATP2C1-201 ENST00000328560.12 3484 888aa Protein coding CCDS46913 P98194-2 - TSL:1, GENCODE basic,
ATP2C1-221 ENST00000513801.5 3439 923aa Protein coding CCDS56280 P98194-3 - TSL:1, GENCODE basic,
ATP2C1-202 ENST00000359644.7 3401 949aa Protein coding CCDS33856 P98194-9 - TSL:1, GENCODE basic,
ATP2C1-203 ENST00000422190.6 3386 939aa Protein coding CCDS46912 P98194-5 - TSL:1, GENCODE basic,
ATP2C1-207 ENST00000504612.5 3148 903aa Protein coding - H0Y9V7 - CDS 5' incomplete, TSL:2,
ATP2C1-215 ENST00000508660.5 943 314aa Protein coding - H0Y9S7 - CDS 5' and 3' incomplete, TSL:5,
ATP2C1-223 ENST00000515854.5 561 131aa Protein coding - D6R9U9 - CDS 3' incomplete, TSL:4,
ATP2C1-217 ENST00000509662.5 559 102aa Protein coding - D6REJ1 - CDS 3' incomplete, TSL:4,
ATP2C1-209 ENST00000505072.5 556 120aa Protein coding - D6RHV9 - CDS 3' incomplete, TSL:4,
ATP2C1-213 ENST00000508297.2 540 82aa Protein coding - D6RGE9 - CDS 3' incomplete, TSL:4,
ATP2C1-206 ENST00000504571.5 786 215aa Nonsense mediated decay - H0Y8X9 - CDS 5' incomplete, TSL:3,
ATP2C1-211 ENST00000507194.1 651 50aa Nonsense mediated decay - H0YAH2 - CDS 5' incomplete, TSL:3,
ATP2C1-216 ENST00000509150.1 573 No protein Processed transcript - - - TSL:3,
ATP2C1-222 ENST00000514654.5 4730 No protein Retained intron - - - TSL:1,
ATP2C1-220 ENST00000513636.1 590 No protein Retained intron - - - TSL:4,
ATP2C1-219 ENST00000510774.1 555 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ATP2C1
This KO Strategy loading
Red Cotton™ Notes Gene ATP2C1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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