CRISPR-U™ Gene Knockout Cell Line Strategy

NPTN Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human NPTN Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NPTN
Gene id 27020
Organism Homo sapiens
Official full symbol neuroplastin
Gene type protein-coding
Also known as GP55, GP65, SDFR1, SDR1, np55, np65
Summary This gene encodes a type I transmembrane protein belonging to the Ig superfamily. The protein is believed to be involved in cell-cell interactions or cell-substrate interactions. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 15
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NPTN-202 ENST00000351217.10 2817 282aa Protein coding CCDS10250 Q9Y639-1 - TSL:1, GENCODE basic,
NPTN-201 ENST00000345330.9 2420 398aa Protein coding CCDS10249 Q9Y639-2 NM_012428.4 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
NPTN-205 ENST00000563691.5 1214 394aa Protein coding CCDS58380 Q9Y639-5 - TSL:1, GENCODE basic, APPRIS ALT2,
NPTN-204 ENST00000562924.5 1094 278aa Protein coding CCDS58379 Q9Y639-3 - TSL:1, GENCODE basic,
NPTN-208 ENST00000565282.5 1247 308aa Protein coding - Q9UFM8 - CDS 3' incomplete, TSL:1,
NPTN-209 ENST00000565325.5 858 184aa Protein coding - H3BU51 - TSL:2,
NPTN-210 ENST00000567198.1 571 152aa Protein coding - H3BQ94 - CDS 3' incomplete, TSL:4,
NPTN-207 ENST00000564551.1 586 No protein Processed transcript - - - TSL:3,
NPTN-206 ENST00000563753.1 599 No protein Retained intron - - - TSL:2,
NPTN-203 ENST00000561898.1 447 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NPTN
This KO Strategy loading
Red Cotton™ Notes Gene NPTN had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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