CRISPR-U™ Gene Knockout Cell Line
Strategy
NPTN Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NPTN Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NPTN |
| Gene id | 27020 |
| Organism | Homo sapiens |
| Official full symbol | neuroplastin |
| Gene type | protein-coding |
| Also known as | GP55, GP65, SDFR1, SDR1, np55, np65 |
| Summary | This gene encodes a type I transmembrane protein belonging to the Ig superfamily. The protein is believed to be involved in cell-cell interactions or cell-substrate interactions. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 15 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NPTN-202 | ENST00000351217.10 | 2817 | 282aa | Protein coding | CCDS10250 | Q9Y639-1 | - | TSL:1, GENCODE basic, |
| NPTN-201 | ENST00000345330.9 | 2420 | 398aa | Protein coding | CCDS10249 | Q9Y639-2 | NM_012428.4 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| NPTN-205 | ENST00000563691.5 | 1214 | 394aa | Protein coding | CCDS58380 | Q9Y639-5 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| NPTN-204 | ENST00000562924.5 | 1094 | 278aa | Protein coding | CCDS58379 | Q9Y639-3 | - | TSL:1, GENCODE basic, |
| NPTN-208 | ENST00000565282.5 | 1247 | 308aa | Protein coding | - | Q9UFM8 | - | CDS 3' incomplete, TSL:1, |
| NPTN-209 | ENST00000565325.5 | 858 | 184aa | Protein coding | - | H3BU51 | - | TSL:2, |
| NPTN-210 | ENST00000567198.1 | 571 | 152aa | Protein coding | - | H3BQ94 | - | CDS 3' incomplete, TSL:4, |
| NPTN-207 | ENST00000564551.1 | 586 | No protein | Processed transcript | - | - | - | TSL:3, |
| NPTN-206 | ENST00000563753.1 | 599 | No protein | Retained intron | - | - | - | TSL:2, |
| NPTN-203 | ENST00000561898.1 | 447 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NPTN |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NPTN had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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